Improved gas chromatography-flame ionization detector analytical method for the analysis of epoxy fatty acids
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Date
2014
Journal Title
Journal ISSN
Volume Title
Publisher
Clinical and Vaccine Immunolog
Abstract
In this study an improved method for analysis of epoxy fatty acids is reported. Data obtained from analysis
of polar fatty acids has previously been presented, but due to the high number of compounds that co-
elute in the polar fraction, the resultant chromatograms are complex which may lead to compromising
the accuracy of the data. A three steps separation of fatty acid methyl esters (FAMEs) by solid-phase
extraction (SPE) on a silica gel column to remove hydroxy fatty acid interferences was proposed. This
approach is opposed to a two step separation procedure that has been often used to prevent analytical
interferences caused by non-altered fatty acids. A gas chromatograph with a flame ionization detector
(GC-FID) equipped with a polar CP-Sil 88TM column was used. Quantification was based on the use of
methyl nonadecanoate (C19:0), as an internal standard. Individual mono epoxy fatty acids were well
separated without co-eluting compounds. The optimized method was finally applied to screen epoxy
fatty acids in 37 fresh oil samples. Results obtained for the total epoxy fatty acids were in the range
0.03ā2 mg gā1 of oil with repeatability coefficient of variation (CV) ranging from 2.8 to 9.9% for duplicate
analysis showing that the results obtained are repeatable.
Description
Keywords
Lipid oxidation, Epoxy fatty acids, Oxygenated polar fatty acids, GC-FID FAMEs
Citation
Gaensbauer, J. T., Rakhola, J. T., Onyango-Makumbi, C., Mubiru, M., Westcott, J. E., Krebs, N. F., ... & Janoff, E. N. (2014). Impaired haemophilus influenzae type b transplacental antibody transmission and declining antibody avidity through the first year of life represent potential vulnerabilities for HIV-exposed but-uninfected infants. Clinical and Vaccine Immunology, 21(12), 1661-1667. http://dx.doi.org/10.1016/j.chroma.2013.10.025