Improved gas chromatography-flame ionization detector analytical method for the analysis of epoxy fatty acids

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dc.contributor.authorMubiru, Edward
dc.contributor.authorShrestha, Kshitij
dc.contributor.authorPapastergiadis, Antonios
dc.contributor.authorDe Meulenaer, Bruno
dc.date.accessioned2023-06-15T21:06:25Z
dc.date.available2023-06-15T21:06:25Z
dc.date.issued2014
dc.description.abstractIn this study an improved method for analysis of epoxy fatty acids is reported. Data obtained from analysis of polar fatty acids has previously been presented, but due to the high number of compounds that co- elute in the polar fraction, the resultant chromatograms are complex which may lead to compromising the accuracy of the data. A three steps separation of fatty acid methyl esters (FAMEs) by solid-phase extraction (SPE) on a silica gel column to remove hydroxy fatty acid interferences was proposed. This approach is opposed to a two step separation procedure that has been often used to prevent analytical interferences caused by non-altered fatty acids. A gas chromatograph with a flame ionization detector (GC-FID) equipped with a polar CP-Sil 88TM column was used. Quantification was based on the use of methyl nonadecanoate (C19:0), as an internal standard. Individual mono epoxy fatty acids were well separated without co-eluting compounds. The optimized method was finally applied to screen epoxy fatty acids in 37 fresh oil samples. Results obtained for the total epoxy fatty acids were in the range 0.03ā€“2 mg gāˆ’1 of oil with repeatability coefficient of variation (CV) ranging from 2.8 to 9.9% for duplicate analysis showing that the results obtained are repeatable.en_US
dc.identifier.citationGaensbauer, J. T., Rakhola, J. T., Onyango-Makumbi, C., Mubiru, M., Westcott, J. E., Krebs, N. F., ... & Janoff, E. N. (2014). Impaired haemophilus influenzae type b transplacental antibody transmission and declining antibody avidity through the first year of life represent potential vulnerabilities for HIV-exposed but-uninfected infants. Clinical and Vaccine Immunology, 21(12), 1661-1667. http://dx.doi.org/10.1016/j.chroma.2013.10.025en_US
dc.identifier.otherhttp://dx.doi.org/10.1016/j.chroma.2013.10.025
dc.identifier.urihttps://nru.uncst.go.ug/handle/123456789/8938
dc.language.isoenen_US
dc.publisherClinical and Vaccine Immunologen_US
dc.subjectLipid oxidationen_US
dc.subjectEpoxy fatty acidsen_US
dc.subjectOxygenated polar fatty acidsen_US
dc.subjectGC-FID FAMEsen_US
dc.titleImproved gas chromatography-flame ionization detector analytical method for the analysis of epoxy fatty acidsen_US
dc.typeArticleen_US
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