Solid-State Deuterium NMR Studies Reveal μs-ns Motions in the HIV-1 TAR RNA Recognition Site
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Date
2008
Journal Title
Journal ISSN
Volume Title
Publisher
Journal of the American Chemical Society
Abstract
Solution and solid-state NMR measurements were used together to examine motion in three sites in
the HIV-1 TAR RNA. We wished to investigate the dynamics facilitating the conformational
rearrangements the TAR RNA must undergo for tat binding, and in particular to characterize the full
range of motional timescales accessible to this RNA. Our results demonstrate that the dynamics in
TAR involving residues essential to tat binding include not only the faster motions detected by
solution relaxation measurements, but also a significant component in the μs-ns timescale.
Description
Keywords
Solid-State Deuterium NMR Studies, μs-ns Motions, HIV-1 TAR RNA, Recognition Site
Citation
Olsen, GL, Echodu, DC, Shajani, Z., Bardaro, MF, Varani, G., & Drobny, GP (2008). Solid-state deuterium NMR studies reveal μs-ns motions in the HIV-1 transactivation response RNA recognition site. Journal of the American Chemical Society, doi:10.1021/ja0778803.