Micro-Perfusion Flow Cell for Imaging Cultured Cells

Agnihotri, Naveen; Kisaalita, William S.; Keith, Charles H.

Micro-Perfusion Flow Cell for Imaging Cultured Cells

Files

Micro-Perfusion Flow.pdf (97.41 KB)

Date

1999

Authors

Agnihotri, Naveen

Kisaalita, William S.

Keith, Charles H.

Publisher

Biotechniques

Abstract

We present a unique design for a flow cell with a small working volume that allows rapid displacement of media viewed under high power and short working distance objectives. The flow cell has a small internal depth (ca. 0.033 cm) and volume (ca. 0.05 mL) and is easy to handle. Made of Delrin®, the flow cell is biologically inert. We have used the flow cell for fluorescence imaging of PC12 cells loaded with tetramethylrhodamine dextran (TMRD) and other dyes.

Citation

Agnihotri, N., Kisaalita, W. S., & Keith, C. H. (1999). Micro-perfusion flow cell for imaging cultured cells. Biotechniques, 27(4), 722-728.https://doi.org/10.2144/99274st01

URI

https://doi.org/10.2144/99274st01
https://nru.uncst.go.ug/handle/123456789/10808

Collections

Engineering and Technology

Full item page