Micro-Perfusion Flow Cell for Imaging Cultured Cells

dc.contributor.authorAgnihotri, Naveen
dc.contributor.authorKisaalita, William S.
dc.contributor.authorKeith, Charles H.
dc.date.accessioned2025-04-16T11:27:34Z
dc.date.available2025-04-16T11:27:34Z
dc.date.issued1999
dc.description.abstractWe present a unique design for a flow cell with a small working volume that allows rapid displacement of media viewed under high power and short working distance objectives. The flow cell has a small internal depth (ca. 0.033 cm) and volume (ca. 0.05 mL) and is easy to handle. Made of Delrin®, the flow cell is biologically inert. We have used the flow cell for fluorescence imaging of PC12 cells loaded with tetramethylrhodamine dextran (TMRD) and other dyes.
dc.identifier.citationAgnihotri, N., Kisaalita, W. S., & Keith, C. H. (1999). Micro-perfusion flow cell for imaging cultured cells. Biotechniques, 27(4), 722-728.https://doi.org/10.2144/99274st01
dc.identifier.urihttps://doi.org/10.2144/99274st01
dc.identifier.urihttps://nru.uncst.go.ug/handle/123456789/10808
dc.language.isoen
dc.publisherBiotechniques
dc.titleMicro-Perfusion Flow Cell for Imaging Cultured Cells
dc.typeArticle
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