Antibacterial Activity of Carica papaya against Methicillin resistant Staphylococcus epidermidis Isolated from Wards Surfaces of Kampala International University Teaching Hospital, Bushenyi, Uganda
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Date
2019
Journal Title
Journal ISSN
Volume Title
Publisher
Current Trends in Biotechnology and Pharmacy
Abstract
This study aimed at determining the
antibacterial activity of Carica papaya against
Methicillin resistant Staphylococcus epidermidis
isolated from doorknobs, bed rails, floors and walls
of Surgical, Medical, Maternity, Pediatrics,
Accident and Emergency, Semiprivate and Private
wards of Kampala International University Teaching
Hospital. The bacteria were isolated from the
wards surfaces and identified using biochemical
tests, Desferroxiomine and Fosfomycin
antibiotics. Disc diffusion method was used to
detect methicillin resistance in S. epidermidis
using Cefoxitin (30 μg) disc. Fresh leaves and
seeds of C. papaya was processed and extracted
using standard methods. Antibacterial activities
of the methanol, acetone and aqueous crude
extracts were assayed using the agar well diffusion
method. Phytochemical analysis, Minimum
Inhibitory and Bactericidal concentration of the
crude extracts were determined using broth
dilution methods. Both C. papaya leaf and seed
crude extract exhibited antibacterial activity
against MRSE with MICs and MBCs ranges of
250 to 31.3mg/ml and 125 to 31.3 mg/mL for leaf
and seed extracts respectively. This study
concludes that C. papaya leaf and seed crude
extracts were effective against Methicillin resistant
S. epidermidis.
Description
Keywords
Antibacterial Activity, Carica papaya, Methicillin, Staphylococcus epidermidis, Kampala International University Teaching Hospital
Citation
Adam, A. S., Ntulume, I., Adeyemo, R., Akinola, S., Abubakar, I. J., Aleiro, A. A., ... & Namatovu, A. (2019). Antibacterial Activity of Carica papaya against Methicillin resistant Staphylococcus epidermidis Isolated from Wards Surfaces of Kampala International University TeachingHospital, Bushenyi, Uganda. Current Trends in Biotechnology and Pharmacy, 13(4), 391-400.