Development of a blocking latex agglutination test for the detection ofantibodies to chicken anemia virus
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Date
2015
Journal Title
Journal ISSN
Volume Title
Publisher
Journal of virological methods
Abstract
tA blocking latex agglutination test (b-LAT) developed in this study was evaluated for the detection ofantibodies against chicken anemia virus (CAV) in chickens. Polystyrene latex beads were coupled witha neutralizing monoclonal antibody (mAb) to CAV (mAb-beads). When mAb-beads were mixed withantigens prepared from the lysate of MDCC-MSB1 cells infected with CAV, agglutination occurred. A shortpre-incubation of CAV antigens with CAV-specific antiserum inhibited the agglutination of mAb-beads.The test results were obtained within 5 min. The specificity of b-LAT was evaluated using sera from specificpathogen-free chickens and sera containing antibodies to avian influenza virus, Newcastle disease virus,infectious bursal disease virus, and Marek’s disease virus; nonspecific agglutination and cross-reactivitywith antibodies to unrelated viruses were not observed. The examination of 94 serum samples collectedfrom commercial breeder chickens of various ages (17–63 weeks) revealed good agreement (93.6%, Kappavalue = 0.82) between b-LAT and a virus neutralization test, known to be most sensitive and specific inthe detection of antibodies to CAV. These results indicate that b-LAT, a simple and rapid test, is a usefuland reliable tool in CAV serology.
Description
Keywords
Chicken anemia virus, Serum antibody, Blocking latex agglutination test, Monoclonal antibody
Citation
Ogawa, H., Bui, V. N., Nguyen, T. T. H., Gronsang, D., Baatartsogt, T., Kizito, M. K., ... & Imai, K. (2015). Development of a blocking latex agglutination test for the detection of antibodies to chicken anemia virus. Journal of virological methods, 221, 74-80. http://dx.doi.org/10.1016/j.jviromet.2015.04.027