Development of a blocking latex agglutination test for the detection ofantibodies to chicken anemia virus

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dc.contributor.authorTrinha, Dai Quang
dc.contributor.authorOgawa, Haruko
dc.contributor.authorNghia Bui, Vuong
dc.contributor.authorThi Hong Nguyen, Tham
dc.contributor.authorGronsang, Dulyatad
dc.contributor.authorBaatartsogt, Tugsbaatar
dc.contributor.authorKahoza Kizito, Mugimba
dc.contributor.authorAbo Elkhair, Mohammed
dc.contributor.authorYamaguchi, Shigeo
dc.contributor.authorNguyen, Viet Khong
dc.contributor.authorImai, Kunitoshi
dc.date.accessioned2023-01-24T19:06:27Z
dc.date.available2023-01-24T19:06:27Z
dc.date.issued2015
dc.description.abstracttA blocking latex agglutination test (b-LAT) developed in this study was evaluated for the detection ofantibodies against chicken anemia virus (CAV) in chickens. Polystyrene latex beads were coupled witha neutralizing monoclonal antibody (mAb) to CAV (mAb-beads). When mAb-beads were mixed withantigens prepared from the lysate of MDCC-MSB1 cells infected with CAV, agglutination occurred. A shortpre-incubation of CAV antigens with CAV-specific antiserum inhibited the agglutination of mAb-beads.The test results were obtained within 5 min. The specificity of b-LAT was evaluated using sera from specificpathogen-free chickens and sera containing antibodies to avian influenza virus, Newcastle disease virus,infectious bursal disease virus, and Marek’s disease virus; nonspecific agglutination and cross-reactivitywith antibodies to unrelated viruses were not observed. The examination of 94 serum samples collectedfrom commercial breeder chickens of various ages (17–63 weeks) revealed good agreement (93.6%, Kappavalue = 0.82) between b-LAT and a virus neutralization test, known to be most sensitive and specific inthe detection of antibodies to CAV. These results indicate that b-LAT, a simple and rapid test, is a usefuland reliable tool in CAV serology.en_US
dc.identifier.citationOgawa, H., Bui, V. N., Nguyen, T. T. H., Gronsang, D., Baatartsogt, T., Kizito, M. K., ... & Imai, K. (2015). Development of a blocking latex agglutination test for the detection of antibodies to chicken anemia virus. Journal of virological methods, 221, 74-80. http://dx.doi.org/10.1016/j.jviromet.2015.04.027en_US
dc.identifier.urihttp://dx.doi.org/10.1016/j.jviromet.2015.04.027
dc.identifier.urihttps://nru.uncst.go.ug/handle/123456789/7185
dc.language.isoenen_US
dc.publisherJournal of virological methodsen_US
dc.subjectChicken anemia virusen_US
dc.subjectSerum antibodyen_US
dc.subjectBlocking latex agglutination testen_US
dc.subjectMonoclonal antibodyen_US
dc.titleDevelopment of a blocking latex agglutination test for the detection ofantibodies to chicken anemia virusen_US
dc.typeArticleen_US
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