Assessment of Murine Neuroblastoma (N1E-115) Resting Membrane Potential by Confocal Microscopy
Loading...
Date
1996
Journal Title
Journal ISSN
Volume Title
Publisher
Journal of Fluorescence
Abstract
Digital imaging (confocal microscopy) and a slow potentiometric dye (tetramethylrhodamine methyl ester) were used to assess the resting membrane potential (V m) of murine neuroblastoma cells (N1E-115). The averageV m was found to be −64.0±2.0 mV. The difference between this and the previously reported higher values was attributed to the use of glass microelectrode techniques that probably caused mechanical injury to the cell membranes: Digital imaging of N1E-115V m was found to be sensitive, reproducible, fast, and simple.
Description
Keywords
Citation
Hernandez, M., Kisaalita, W. S., & Farmer, M. A. (1996). Assessment of murine neuroblastoma (N1E-115) resting membrane potential by confocal microscopy. Journal of Fluorescence, 6, 77-82.