Determination of the G and P Types of Previously Nontypeable Rotavirus Strains from the African Rotavirus Network, 1996–2004: Identification of Unusual G Types
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Date
2010
Journal Title
Journal ISSN
Volume Title
Publisher
Journal of Infectious Diseases
Abstract
A total of 215 nontypeable rotavirus samples collected from children <5 years of age by members of the African Rotavirus Network were characterized using reverse-transcription polymerase chain reaction analysis and sequencing. The most predominant strain identified was P[8]G1 (46.9%). Genotypes P[8]G10, P[8]G8, P[6]G8, and P[7]G5 were also detected at frequencies varying from 0.5% to 2.3%. This study suggests that reassortment of unusual G types into a background of globally common genotype P[8] strains may be a major mechanism of generating rotavirus diversity. Nucleotide substitutions at the P[8], P[6], and G1 primer binding sites accounted for the failure to type these strains initially. Hence, these findings highlight the need for regular evaluation of rotavirus genotyping methods.
Description
Keywords
polymerase chain reaction, binding sites, genotype, genotype determination
Citation
Esona, M. D., Steele, D., Kerin, T., Armah, G., Peenze, I., Geyer, A., ... & Gentsch, J. (2010). Determination of the G and P types of previously nontypeable rotavirus strains from the African Rotavirus Network, 1996–2004: identification of unusual G types. Journal of Infectious Diseases, 202(Supplement_1), S49-S54.https://doi.org/10.1086/653552