Determination of cyanobacterial cyclic peptide hepatotoxins in drinking water using CE
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Date
2009
Authors
Journal Title
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Publisher
Electrophoresis
Abstract
Four cyanobacteria hepatotoxins microcystin LR, microcystin RR, microcystin YR, and
nodularin were simultaneously determined in drinking water using CZE and MEKC
coupled with UV detection. The toxins were satisfactorily separated in both CZE and
MEKC modes. Detection limits were in the range of 0.82–4.81 mg/mL, with R2 values of
0.994–0.999. The linearity range tested for the standards was 5–100 mg/mL and RSD
percentages were in the range of 1.0–2.5% for retention time and 3.0–10.2% for peak
area. When a known amount of standard was spiked into a known volume of water and
extracted, recoveries were 90.3% (RR), 101.5% (nodularin), 90.6% (YR), and 88.2% (LR).
The use of SPE enabled cleanup and pre-concentration of a real sample to achieve a 100-
fold concentration factor. Detection limits after SPE of the real sample spiked with
microcystins were 0.090 mg/L (RR), 0.076 mg/L (YR), and 0.110 mg/L (LR), with RSD
percentage values of 9.9–11.7% for peak area and 2.2–3.3% for retention time. The
technique developed provides an alternative method for determining microcystins at
levels of concentration that will be able to meet WHO drinking water guidelines for
microcystins.
Description
Keywords
CE, Microcystin(s), Nodularin
Citation
Birungi, G., & Yau Li, S. F. (2009). Determination of cyanobacterial cyclic peptide hepatotoxins in drinking water using CE. Electrophoresis, 30(15), 2737-2742. DOI 10.1002/elps.200900030