Neural Cell 3D Microtissue Formation Is Marked by Cytokines’ Up-Regulation

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dc.contributor.authorLai, Yinzhi
dc.contributor.authorAsthana, Amish
dc.contributor.authorKisaalita, William S.
dc.date.accessioned2025-04-16T12:02:49Z
dc.date.available2025-04-16T12:02:49Z
dc.date.issued2011
dc.description.abstractCells cultured in three dimensional (3D) scaffolds as opposed to traditional two-dimensional (2D) substrates have been considered more physiologically relevant based on their superior ability to emulate the in vivo environment. Combined with stem cell technology, 3D cell cultures can provide a promising alternative for use in cell-based assays or biosensors in non-clinical drug discovery studies. To advance 3D culture technology, a case has been made for identifying and validating three-dimensionality biomarkers. With this goal in mind, we conducted a transcriptomic expression comparison among neural progenitor cells cultured on 2D substrates, 3D porous polystyrene scaffolds, and as 3D neurospheres (in vivo surrogate). Up-regulation of cytokines as a group in 3D and neurospheres was observed. A group of 13 cytokines were commonly up-regulated in cells cultured in polystyrene scaffolds and neurospheres, suggesting potential for any or a combination from this list to serve as three-dimensionality biomarkers. These results are supportive of further cytokine identification and validation studies with cells from non-neural tissue.
dc.identifier.citationLai, Y., Asthana, A., Cheng, K., & Kisaalita, W. S. (2011). Neural cell 3D microtissue formation is marked by cytokines' up-regulation. PLoS One, 6(10), e26821.https://doi.org/10.1371/journal.pone.0026821
dc.identifier.urihttps://doi.org/10.1371/journal.pone.0026821
dc.identifier.urihttps://nru.uncst.go.ug/handle/123456789/10813
dc.language.isoen
dc.publisherPLoS One
dc.titleNeural Cell 3D Microtissue Formation Is Marked by Cytokines’ Up-Regulation
dc.typeArticle
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