CCR5 promoter variants among Ugandan HIV-1 elite and viremic controllers: a laboratory ฀ based cross ฀ sectional study

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dc.contributor.authorNyiro, Brian
dc.contributor.authorAmanya, Sharon B.
dc.contributor.authorNabatanzi, Rose
dc.contributor.authorBayiyana, Alice
dc.contributor.authorKalazane, Linda I.
dc.contributor.authorWaswa, Francis
dc.contributor.authorNabulime, Eva
dc.contributor.authorKarara, Daniel
dc.contributor.authorKabali, Joel
dc.contributor.authorMboowa, Gerald
dc.contributor.authorKayongo, Alex
dc.contributor.authorKateete, David P.
dc.contributor.authorNankya, Immaculate
dc.date.accessioned2022-12-01T01:18:09Z
dc.date.available2022-12-01T01:18:09Z
dc.date.issued2020
dc.description.abstractMechanisms for HIV control among HIV-1 elite and viremic-controllers are not fully understood. In Uganda, Studies have reported individuals who without Antiretroviral therapy have the inherent ability to control HIV progression to AIDS for a period of greater than 5 years. However, reasons for this phenotype are not understood. The study objective was to determine the distribution of CCR5 co-receptor on CD4+ T-cells and its associated promoter variants among HIV-1 elite and viremic-controllers. Methods We isolated CD4+T-cells from PBMCs using EasySep CD4+ T-cell negative selection kit, and stimulated them with anti-CD3 and anti-CD28 for 48 hours. To quantify CCR5 expression, we performed immune-phenotyping using flow cytometry. CCR5 promoter polymorphisms were determined through sanger sequencing. The Kruskal–Wallis and the Mann-Whitney test were used to compare differences in the percentages of CCR5+ CD4+ T-cells and the differences in CCR5 densities on CD4+ T-cells respectively. p values < 0.05 were considered significant. Results The percentage of CCR5+CD4+ T-cells was higher among the non-controllers compared to the controllers although, the difference was not statistically significant; elite and viremic-controllers (p=0.9173), viremic and non-controllers (0.0702), elite and non-controllers (0.6010). Of significance was the CCR5 densities on CD4+ T-cells, which were significantly higher among non-controllers relative to the controllers; elite and viremic-controllers (p=3048), viremic and non43 controllers (P=0.0312), elite and non-controllers (P=0.0210)en_US
dc.identifier.citationNyiro, B., Amanya, S. B., Nabatanzi, R., Bayiyana, A., Kalazane, L. I., Waswa, F., ... & Sande, O. J. (2020). CCR5 promoter variants among Ugandan HIV-1 elite and viremic controllers: a laboratory‑based cross‑sectional study.https://orcid.org/0000-0001-7131-8137en_US
dc.identifier.otherhttps://orcid.org/0000-0001-7131-8137
dc.identifier.urihttps://nru.uncst.go.ug/handle/123456789/5604
dc.language.isoenen_US
dc.publisherResearch Squareen_US
dc.subjectCCR5en_US
dc.subjectHIVen_US
dc.subjectElite controllersen_US
dc.subjectViremic controllers and non-controllersen_US
dc.titleCCR5 promoter variants among Ugandan HIV-1 elite and viremic controllers: a laboratory ฀ based cross ฀ sectional studyen_US
dc.typeArticleen_US
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