CCR5 promoter variants among Ugandan HIV-1 elite and viremic controllers: a laboratory based cross sectional study
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Date
2020
Journal Title
Journal ISSN
Volume Title
Publisher
Research Square
Abstract
Mechanisms for HIV control among HIV-1 elite and viremic-controllers are not fully understood. In Uganda,
Studies have reported individuals who without Antiretroviral therapy have the inherent ability to control HIV
progression to AIDS for a period of greater than 5 years. However, reasons for this phenotype are not
understood. The study objective was to determine the distribution of CCR5 co-receptor on CD4+ T-cells and
its associated promoter variants among HIV-1 elite and viremic-controllers.
Methods
We isolated CD4+T-cells from PBMCs using EasySep CD4+ T-cell negative selection kit, and
stimulated them with anti-CD3 and anti-CD28 for 48 hours. To quantify CCR5 expression, we
performed immune-phenotyping using flow cytometry. CCR5 promoter polymorphisms were
determined through sanger sequencing. The Kruskal–Wallis and the Mann-Whitney test were used to
compare differences in the percentages of CCR5+ CD4+ T-cells and the differences in CCR5
densities on CD4+ T-cells respectively. p values < 0.05 were considered significant.
Results
The percentage of CCR5+CD4+ T-cells was higher among the non-controllers compared to the
controllers although, the difference was not statistically significant; elite and viremic-controllers
(p=0.9173), viremic and non-controllers (0.0702), elite and non-controllers (0.6010). Of
significance was the CCR5 densities on CD4+ T-cells, which were significantly higher among
non-controllers relative to the controllers; elite and viremic-controllers (p=3048), viremic and non43
controllers (P=0.0312), elite and non-controllers (P=0.0210)
Description
Keywords
CCR5, HIV, Elite controllers, Viremic controllers and non-controllers
Citation
Nyiro, B., Amanya, S. B., Nabatanzi, R., Bayiyana, A., Kalazane, L. I., Waswa, F., ... & Sande, O. J. (2020). CCR5 promoter variants among Ugandan HIV-1 elite and viremic controllers: a laboratory‑based cross‑sectional study.https://orcid.org/0000-0001-7131-8137