Operationalization of COVID-19 Rapid Diagnosis Using Xpert® Xpress SARS CoV-2 Assay in Resource-Limited Settings: Early Implementation Lessons From Uganda

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Date
2021
Authors
Nsawotebba, Andrew
Ibanda, Ivan
Ssentalo Bagaya, Bernard
Nyombi, Abdunoor
Kagirita, Atek
Tugumisirize, Didas
Mujuni, Dennis
Majwala, Robert Kaos
Ocen, Francis
Kabugo, Joel
Journal Title
Journal ISSN
Volume Title
Publisher
Research Square
Abstract
The novel Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) that causes COVID-19 disease is a global challenge. Several countries have adopted testing, isolation, and tracing strategy towards the control of the COVID-19 pandemic, but access to rapid and accurate testing is still a global challenge. The conventional PCR – based assay is the most commonly used test yet it has huge costs, infrastructural, and procurement logistical challenges. The Xpert® Xpress SARS-CoV-2 test is an automated in – vitro diagnostic test for the qualitative detection of nucleic acid from SARS-CoV-2 within a turnaround time of 60 minutes on the widely used GeneXpert Dx Instrument Systems. Here we document the best practices and challenges encountered with the operationalization of Xpert® Xpress SARS-CoV-2 testing in a resource-limited setting. Materials and Methods: The Xpert® Xpress SARS-CoV-2 implementation followed an operational work plan that included; Laboratory COVID-19 policy and planning, situational analysis of the Laboratory network, country Xpert® Xpress SARS-CoV-2 assay verification, and rollout at Mutukula Port Health Laboratory. The Laboratory strategy was based on a set of six objectives; conducting infrastructural modifications, building a strong COVID-19 testing capacity, developing robust Laboratory Quality and Information Management Systems, establishing a Bio-risk management and Bio-banking capacity.
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Keywords
COVID-19, Xpert® Xpress SARS CoV-2, Rapid diagnosis, Resource-Limited Settings, Implementation
Citation
Nsawotebba, A., Ibanda, I., Ssewanyana, I., Bagaya, B. S., Nyombi, A., Kagirita, A., ... & Ssengooba, W. (2021). Operationalization of COVID-19 Rapid Diagnosis Using Xpert® Xpress SARS CoV-2 Assay in Resource-Limited Settings: Early Implementation Lessons From Uganda. https://doi.org/10.21203/rs.3.rs-154102/v1