Cervicovaginal Bacteriology and Antibiotic Sensitivity Patterns among Women with Premature Rupture of Membranes in Mulago Hospital, Kampala, Uganda: A Cross-Sectional Study
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Date
2017
Journal Title
Journal ISSN
Volume Title
Publisher
Infectious diseases in obstetrics and gynecology
Abstract
A 2013 Cochrane review concluded that the choice of antibiotics for prophylaxis in PROM is not clear. In Uganda,
a combination of oral erythromycin and amoxicillin is the 1st line for prophylaxis against ascending infection. Our aim was to
establish the current cervicovaginal bacteriology and antibiotic sensitivity patterns. Methods. Liquor was collected aseptically from
the endocervical canal and pool in the posterior fornix of the vagina using a pipette. Aerobic cultures were performed on blood,
chocolate, and MacConkey agar and incubated at 35โ37โC for 24โ48 hrs. Enrichment media were utilized to culture for GBS and
facultative anaerobes. Isolates were identified using colonial morphology, gram staining, and biochemical analysis. Sensitivity
testing was performed via Kirby-Bauer disk diffusion and dilution method. Pearsonโs chi-squared (๐2) test and the paired t-test
were applied, at a ๐ value of 0.05. Results. Thirty percent of the cultures were positive and over 90% were aerobic microorganisms.
Resistance to erythromycin, ampicillin, cotrimoxazole, and ceftriaxone was 44%, 95%, 96%, and 24%, respectively. Rupture of
membranes (>12 hrs), late preterm, and term PROM were associated with more positive cultures. Conclusion. The spectrum of
bacteria associated with PROM has not changed, but resistance to erythromycin and ampicillin has increased.
Description
Keywords
Cervicovaginal Bacteriology, Antibiotic Sensitivity Patterns, Women, Premature Rupture, Membranes
Citation
Musaba, M. W., Kagawa, M. N., Kiggundu, C., Kiondo, P., & Wandabwa, J. (2017). Cervicovaginal bacteriology and antibiotic sensitivity patterns among women with premature rupture of membranes in Mulago hospital, Kampala, Uganda: a cross-sectional study. Infectious diseases in obstetrics and gynecology, 2017. https://doi.org/10.1155/2017/9264571