Whole genome analysis of selected human and animal rotaviruses identified in Uganda from 2012 to 2014 reveals complex genome reassortment events between human, bovine, caprine and porcine strains

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dc.contributor.authorBwogi, Josephine
dc.contributor.authorJere, Khuzwayo C.
dc.contributor.authorKaramagi, Charles
dc.contributor.authorByarugaba, Denis K.
dc.contributor.authorNamuwulya, Prossy
dc.contributor.authorBaliraine, Frederick N.
dc.contributor.authorDesselberger, Ulrich
dc.contributor.authorIturriza-Gomara, Miren
dc.date.accessioned2022-08-05T16:05:47Z
dc.date.available2022-08-05T16:05:47Z
dc.date.issued2017
dc.description.abstractRotaviruses of species A (RVA) are a common cause of diarrhoea in children and the young of various other mammals and birds worldwide. To investigate possible interspecies transmission of RVAs, whole genomes of 18 human and 6 domestic animal RVA strains identified in Uganda between 2012 and 2014 were sequenced using the Illumina HiSeq platform. The backbone of the human RVA strains had either a Wa- or a DS-1-like genetic constellation. One human strain was a Wa-like mono-reassortant containing a DS-1-like VP2 gene of possible animal origin. All eleven genes of one bovine RVA strain were closely related to those of human RVAs. One caprine strain had a mixed genotype backbone, suggesting that it emerged from multiple reassortment events involving different host species. The porcine RVA strains had mixed genotype backbones with possible multiple reassortant events with strains of human and bovine origin.Overall, whole genome characterisation of rotaviruses found in domestic animals in Uganda strongly suggested the presence of human-to animal RVA transmission, with concomitant circulation of multi-reassortant strains potentially derived from complex interspecies transmission events. However, whole genome data from the human RVA strains causing moderate and severe diarrhoea in under-fives in Uganda indicated that they were primarily transmitted from person-to-person.en_US
dc.identifier.citationBwogi J, Jere KC, Karamagi C, Byarugaba DK, Namuwulya P, Baliraine FN, et al. (2017) Whole genome analysis of selected human and animal rotaviruses identified in Uganda from 2012 to 2014 reveals complex genome reassortment events between human, bovine, caprine and porcine strains. PLoS ONE 12(6): e0178855. https://doi.org/10.1371/journal.pone.0178855en_US
dc.identifier.urihttps://doi.org/10.1371/journal.pone.0178855
dc.identifier.urihttps://nru.uncst.go.ug/handle/123456789/4259
dc.language.isoenen_US
dc.publisherPLoS ONEen_US
dc.subjectGenomeen_US
dc.subjectHumanen_US
dc.subjectAnimal rotavirusesen_US
dc.subjectUgandaen_US
dc.subjectBovineen_US
dc.subjectPorcine strainsen_US
dc.titleWhole genome analysis of selected human and animal rotaviruses identified in Uganda from 2012 to 2014 reveals complex genome reassortment events between human, bovine, caprine and porcine strainsen_US
dc.typeArticleen_US
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