Whole genome analysis of selected human and animal rotaviruses identified in Uganda from 2012 to 2014 reveals complex genome reassortment events between human, bovine, caprine and porcine strains
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Date
2017
Journal Title
Journal ISSN
Volume Title
Publisher
PLoS ONE
Abstract
Rotaviruses of species A (RVA) are a common cause of diarrhoea in children and the young
of various other mammals and birds worldwide. To investigate possible interspecies transmission
of RVAs, whole genomes of 18 human and 6 domestic animal RVA strains identified
in Uganda between 2012 and 2014 were sequenced using the Illumina HiSeq platform. The
backbone of the human RVA strains had either a Wa- or a DS-1-like genetic constellation.
One human strain was a Wa-like mono-reassortant containing a DS-1-like VP2 gene of possible
animal origin. All eleven genes of one bovine RVA strain were closely related to those
of human RVAs. One caprine strain had a mixed genotype backbone, suggesting that it
emerged from multiple reassortment events involving different host species. The porcine
RVA strains had mixed genotype backbones with possible multiple reassortant events with
strains of human and bovine origin.Overall, whole genome characterisation of rotaviruses
found in domestic animals in Uganda strongly suggested the presence of human-to animal
RVA transmission, with concomitant circulation of multi-reassortant strains potentially
derived from complex interspecies transmission events. However, whole genome data from
the human RVA strains causing moderate and severe diarrhoea in under-fives in Uganda
indicated that they were primarily transmitted from person-to-person.
Description
Keywords
Genome, Human, Animal rotaviruses, Uganda, Bovine, Porcine strains
Citation
Bwogi J, Jere KC, Karamagi C, Byarugaba DK, Namuwulya P, Baliraine FN, et al. (2017) Whole genome analysis of selected human and animal rotaviruses identified in Uganda from 2012 to 2014 reveals complex genome reassortment events between human, bovine, caprine and porcine strains. PLoS ONE 12(6): e0178855. https://doi.org/10.1371/journal.pone.0178855