Human Herpesvirus Type 8 Variants Circulating In Europe, Africa And North America In Classic, Endemic And Epidemic Kaposi's Sarcoma Lesions During Pre-AIDS and AIDS Era

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Human Herpesvirus Type 8 Variants Circulating In Europe, Africa And North America In Classic, Endemic And Epidemic Kaposi's Sarcoma Lesions During Pre-AIDS and AIDS Era.pdf (937.55 KB)
Date
2010
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Virology
Abstract
Human herpesvirus-8 (HHV-8) variants have been found heterogeneously distributed among human populations living in diverse geographic regions, but their differential pathogenicity in Kaposi's sarcoma development remains controversial. In the present study, HHV-8 variant distribution has been analyzed in classic, iatrogenic, endemic as well as epidemic Kaposi's sarcoma (KS) during pre-AIDS and AIDS period (1971–2008) in countries with different KS incidence rate. DNA samples from cutaneous KS lesions of 68 patients living in Africa (n = 23, Cameroon, Kenya and Uganda), Europe (n = 34, Greece and Italy) and North America (n = 11) have been subjected to PCR amplification of HHV-8 ORF 26, T0.7, K1 and K14.1/15, followed by direct nucleotide sequencing and phylogenetic analysis. Among the 23 African samples, the majority of HHV-8 ORF 26 variants clustered with the subtype R (n = 12) and B (n = 5). Conversely, the viral sequences obtained from 45 European and North European tumors belonged mainly to subtype A/C (n = 36). In general, HHV-8 and K1 variant clustering paralleled that of ORF 26 and T0.7. Genotyping of the K14.1/15 loci revealed a large predominance of P subtype in all tumors. In conclusion, comparison of the HHV-8 sequences from classic or endemic versus AIDS-associated KS showed a strong linkage of the HHV-8 variants with specific populations, which has not changed during AIDS epidemic.
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Keywords
Kaposi's sarcoma; Human herpes virus type 8; (HHV-8)HHV-8 ORF 26; HHV-8 ORF K1
Citation
Tornesello, M. L., Biryahwaho, B., Downing, R., Hatzakis, A., Alessi, E., Cusini, M., ... & Buonaguro, F. M. (2010). Human herpesvirus type 8 variants circulating in Europe, Africa and North America in classic, endemic and epidemic Kaposi's sarcoma lesions during pre-AIDS and AIDS era. Virology, 398(2), 280-289.https://doi.org/10.1016/j.virol.2009.12.005
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https://nru.uncst.go.ug/xmlui/handle/123456789/1529
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Medical and Health Sciences