Polymerase Chain Reaction Detection of Trypanosoma cruzi in Macaca fascicularis Using Archived Tissues

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dc.contributor.authorWilliams, Jeff T.
dc.contributor.authorMubiru, James N.
dc.contributor.authorSchlabritz-Loutsevitch, Natalia E.
dc.contributor.authorRubicz, Rohina C.
dc.contributor.authorVandeBerg, John L.
dc.contributor.authorDick Jr, Edward J.
dc.contributor.authorHubbard, Gene B.
dc.date.accessioned2023-06-23T17:57:20Z
dc.date.available2023-06-23T17:57:20Z
dc.date.issued2009
dc.description.abstractThis study describes conventional and real-time polymerase chain reaction (PCR) methods developed to detect and quantify Trypanosoma cruzi DNA in cynomolgus monkeys (Macaca fascicularis) using formalin-fixed paraffin-embedded blocks archived for periods of up to 6 years. The highest concentration of T. cruzi DNA was found in the myocardium, urinary bladder, stomach, lymph node, adrenal gland, and colon. The concentration of T. cruzi DNA detected in cardiac tissues was 10–100- fold greater than found elsewhere; the mean concentrations of T. cruzi DNA in non-cardiac tissues were otherwise comparable. Trypanosoma cruzi DNA was amplified from cerebrum but not cerebellum or kidney. Successful use of DNA from formalin-fixed, paraffin-embedded blocks is important because most pathology laboratories routinely archive wax blocks. This archived resource can be used for further studies on the prevalence of this disease.en_US
dc.identifier.citationWilliams, J. T., Mubiru, J. N., Schlabritz-Loutsevitch, N. E., Rubicz, R. C., VandeBerg, J. L., Dick Jr, E. J., & Hubbard, G. B. (2009). Polymerase chain reaction detection of Trypanosoma cruzi in Macaca fascicularis using archived tissues. The American journal of tropical medicine and hygiene, 81(2), 228.en_US
dc.identifier.urihttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2740910/
dc.identifier.urihttps://nru.uncst.go.ug/handle/123456789/8982
dc.language.isoenen_US
dc.publisherThe American journal of tropical medicine and hygieneen_US
dc.subjectPolymerase Chain Reactionen_US
dc.subjectTrypanosoma cruzien_US
dc.subjectMacaca fascicularisen_US
dc.subjectArchived Tissuesen_US
dc.titlePolymerase Chain Reaction Detection of Trypanosoma cruzi in Macaca fascicularis Using Archived Tissuesen_US
dc.typeArticleen_US
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