Polymerase Chain Reaction Detection of Trypanosoma cruzi in Macaca fascicularis Using Archived Tissues
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Date
2009
Journal Title
Journal ISSN
Volume Title
Publisher
The American journal of tropical medicine and hygiene
Abstract
This study describes conventional and real-time polymerase chain reaction (PCR) methods developed
to detect and quantify Trypanosoma cruzi DNA in cynomolgus monkeys (Macaca fascicularis) using
formalin-fixed paraffin-embedded blocks archived for periods of up to 6 years. The highest
concentration of T. cruzi DNA was found in the myocardium, urinary bladder, stomach, lymph node,
adrenal gland, and colon. The concentration of T. cruzi DNA detected in cardiac tissues was 10–100-
fold greater than found elsewhere; the mean concentrations of T. cruzi DNA in non-cardiac tissues
were otherwise comparable. Trypanosoma cruzi DNA was amplified from cerebrum but not
cerebellum or kidney. Successful use of DNA from formalin-fixed, paraffin-embedded blocks is
important because most pathology laboratories routinely archive wax blocks. This archived resource
can be used for further studies on the prevalence of this disease.
Description
Keywords
Polymerase Chain Reaction, Trypanosoma cruzi, Macaca fascicularis, Archived Tissues
Citation
Williams, J. T., Mubiru, J. N., Schlabritz-Loutsevitch, N. E., Rubicz, R. C., VandeBerg, J. L., Dick Jr, E. J., & Hubbard, G. B. (2009). Polymerase chain reaction detection of Trypanosoma cruzi in Macaca fascicularis using archived tissues. The American journal of tropical medicine and hygiene, 81(2), 228.