Effect of Pseudomonas fluorescens (2–79) culture age on the relationship between optical density and biomass

Abstract

The effect of cell-cell adhesion (clump formation) on the relationship between optical density and dry biomass was investigated for Pseudomonas fluorescens (2–79). Calibration curves were generated at the beginning, middle and end of the arithmetic growth phase, as well as during the decay phase. The results show that use of a single biomass-turbidimetric equation for the entire arithmetic growth phase may yield erroneous results.