Multilocus sequence analysis revealed a high genotypic diversity of Aeromonas hydrophila infecting fish in Uganda

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dc.contributor.authorWamala, Samuel Posian
dc.contributor.authorKahoza Mugimba, Kizito
dc.contributor.authorDubey, Saurabh
dc.contributor.authorTakele, Abayneh
dc.contributor.authorMweemba Munang'andu, Hetron
dc.contributor.authorEvensen, Øystein
dc.contributor.authorMutoloki, Stephen
dc.contributor.authorKaruhize Byarugaba, Denis
dc.contributor.authorSørum, Henning
dc.date.accessioned2023-01-24T19:19:37Z
dc.date.available2023-01-24T19:19:37Z
dc.date.issued2018
dc.description.abstractmultilocus sequence analysis (MLSA) was carried out to delineate Aeromonas hydrophila from fish in Uganda. Five housekeeping genes including recA, gyrB, metG, gltA and pps; and the 16S rRNA gene were amplified and sequenced from a total of nine A. hydrophila isolates. The obtained sequences were edited, and consensus sequences generated for each gene locus. The housekeeping gene sequences were concatenated and phylogenetic analysis performed in MEGA version 7.0.2. Pairwise distances ranged from 0.000 to 0.118, highest within the gltA gene locus and lowest within the 16S rRNA gene. The average evolutionary diversity within isolates from the same source ranged between 0.002 and 0.037, and it was 0.033 between the different sources. Similar tree topologies were obtained from the different gene loci with recA, metG and gyrB being more consistent in discriminating isolates according to sources while the 16S rRNA gene had the lowest resolution. The concatenated tree had the highest discriminatory power. This study revealed that A. hydrophila strains infecting fish in Uganda are of diverse genotypes suggesting different sources of infection in a given outbreak. Efforts to minimize spread of the bacteria across sources should be emphasized to control infections of mixed genotypes.en_US
dc.identifier.citationWamala, S. P., Mugimba, K. K., Dubey, S., Takele, A., Munang'andu, H. M., Evensen, Ø., ... & Sørum, H. (2018). Multilocus sequence analysis revealed a high genotypic diversity of Aeromonas hydrophila infecting fish in Uganda. Journal of fish diseases, 41(10), 1589-1600. DOI: 10.1111/jfd.12873en_US
dc.identifier.other10.1111/jfd.12873
dc.identifier.urihttps://nru.uncst.go.ug/handle/123456789/7186
dc.language.isoenen_US
dc.publisherJournal of fish diseasesen_US
dc.subjectAeromonas hydrophilaen_US
dc.subjectFishen_US
dc.subjectGenotypic diversityen_US
dc.subjectMultilocus sequence analysisen_US
dc.titleMultilocus sequence analysis revealed a high genotypic diversity of Aeromonas hydrophila infecting fish in Ugandaen_US
dc.typeArticleen_US
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