Multilocus sequence analysis revealed a high genotypic diversity of Aeromonas hydrophila infecting fish in Uganda
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Date
2018
Journal Title
Journal ISSN
Volume Title
Publisher
Journal of fish diseases
Abstract
multilocus sequence analysis (MLSA) was carried out to delineate Aeromonas
hydrophila from fish in Uganda. Five housekeeping genes including recA, gyrB, metG,
gltA and pps; and the 16S rRNA gene were amplified and sequenced from a total
of nine A. hydrophila isolates. The obtained sequences were edited, and consensus
sequences generated for each gene locus. The housekeeping gene sequences were
concatenated and phylogenetic analysis performed in MEGA version 7.0.2. Pairwise
distances ranged from 0.000 to 0.118, highest within the gltA gene locus and lowest
within the 16S rRNA gene. The average evolutionary diversity within isolates
from the same source ranged between 0.002 and 0.037, and it was 0.033 between
the different sources. Similar tree topologies were obtained from the different gene
loci with recA, metG and gyrB being more consistent in discriminating isolates
according to sources while the 16S rRNA gene had the lowest resolution. The concatenated
tree had the highest discriminatory power. This study revealed that
A. hydrophila strains infecting fish in Uganda are of diverse genotypes suggesting
different sources of infection in a given outbreak. Efforts to minimize spread of
the bacteria across sources should be emphasized to control infections of mixed
genotypes.
Description
Keywords
Aeromonas hydrophila, Fish, Genotypic diversity, Multilocus sequence analysis
Citation
Wamala, S. P., Mugimba, K. K., Dubey, S., Takele, A., Munang'andu, H. M., Evensen, Ø., ... & Sørum, H. (2018). Multilocus sequence analysis revealed a high genotypic diversity of Aeromonas hydrophila infecting fish in Uganda. Journal of fish diseases, 41(10), 1589-1600. DOI: 10.1111/jfd.12873