Characterization and mapping of Dt1 locus which co‑segregates with CcTFL1 for growth habit in pigeonpea

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dc.contributor.authorSaxena, Rachit K.
dc.contributor.authorObala, Jimmy
dc.contributor.authorSinjushin, Andrey
dc.contributor.authorKumar, C.V. Sameer
dc.contributor.authorSaxena, K.B.
dc.contributor.authorVarshney, Rajeev K.
dc.date.accessioned2023-09-06T17:29:05Z
dc.date.available2023-09-06T17:29:05Z
dc.date.issued2017
dc.description.abstractPigeonpea (Cajanus cajan) is one of the most important legume crops grown in arid and semi-arid regions of the world. It is characterized with few unique features compared with other legume species, such as Lotus, Medicago, and Glycine. One of them is growth habit, an important agronomic trait. In the present study, identification of mutations affecting growth habit accompanied by a precise analysis of phenotype has been done which will shed more light upon developmental regulation in pigeonpea. A genetic study was conducted to examine the inheritance of growth habit and a genotyping by sequencing (GBS)-based genetic map constructed using F2 mapping population derived from crossing parents ICP 5529 and ICP 11605. Inheritance studies clearly demonstrated the dominance of indeterminate (IDT) growth habit over determinate (DT) growth habit in F2 and F2: 3 progenies. A total of 787 SNP markers were mapped in the genetic map of 1454 cM map length. Growth habit locus (Dt1) was mapped on the CcLG03 contributing more than 61% of total phenotypic variations. Subsequently, QTL analysis highlighted one gene, CcTFL1, as a candidate for determinacy in pigeonpea, since an Indel marker derived from this gene co-segregated with the Dt1 locus. Ability of this Indel-derived marker to differentiate DT/IDT lines was also validated on 262 pigeonpea lines. This study clearly demonstrated that CcTFL1 is a candidate gene for growth habit in pigeonpea and a user-friendly marker was developed in the present study which will allow low-cost genotyping without need of automation.en_US
dc.identifier.citationSaxena, R. K., Obala, J., Sinjushin, A., Kumar, C. S., Saxena, K. B., & Varshney, R. K. (2017). Characterization and mapping of Dt1 locus which co-segregates with CcTFL1 for growth habit in pigeonpea. Theoretical and Applied Genetics, 130, 1773-1784. DOI 10.1007/s00122-017-2924-2en_US
dc.identifier.other10.1007/s00122-017-2924-2
dc.identifier.urihttps://nru.uncst.go.ug/handle/123456789/9182
dc.language.isoenen_US
dc.publisherTheoretical and Applied Geneticsen_US
dc.subjectMappingen_US
dc.subjectDt1 locusen_US
dc.subjectCcTFL1en_US
dc.subjectPigeonpeaen_US
dc.titleCharacterization and mapping of Dt1 locus which co‑segregates with CcTFL1 for growth habit in pigeonpeaen_US
dc.typeArticleen_US
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