Characterization and mapping of Dt1 locus which co‑segregates with CcTFL1 for growth habit in pigeonpea
Loading...
Date
2017
Journal Title
Journal ISSN
Volume Title
Publisher
Theoretical and Applied Genetics
Abstract
Pigeonpea (Cajanus cajan) is one of the most
important legume crops grown in arid and semi-arid regions
of the world. It is characterized with few unique features
compared with other legume species, such as Lotus, Medicago,
and Glycine. One of them is growth habit, an important
agronomic trait. In the present study, identification of
mutations affecting growth habit accompanied by a precise
analysis of phenotype has been done which will shed
more light upon developmental regulation in pigeonpea. A
genetic study was conducted to examine the inheritance of
growth habit and a genotyping by sequencing (GBS)-based genetic map constructed using F2
mapping population
derived from crossing parents ICP 5529 and ICP 11605.
Inheritance studies clearly demonstrated the dominance
of indeterminate (IDT) growth habit over determinate
(DT) growth habit in F2
and F2:
3 progenies. A total of 787
SNP markers were mapped in the genetic map of 1454 cM
map length. Growth habit locus (Dt1) was mapped on the
CcLG03 contributing more than 61% of total phenotypic
variations. Subsequently, QTL analysis highlighted one
gene, CcTFL1, as a candidate for determinacy in pigeonpea,
since an Indel marker derived from this gene co-segregated
with the Dt1 locus. Ability of this Indel-derived
marker to differentiate DT/IDT lines was also validated on
262 pigeonpea lines. This study clearly demonstrated that
CcTFL1 is a candidate gene for growth habit in pigeonpea
and a user-friendly marker was developed in the present
study which will allow low-cost genotyping without need
of automation.
Description
Keywords
Mapping, Dt1 locus, CcTFL1, Pigeonpea
Citation
Saxena, R. K., Obala, J., Sinjushin, A., Kumar, C. S., Saxena, K. B., & Varshney, R. K. (2017). Characterization and mapping of Dt1 locus which co-segregates with CcTFL1 for growth habit in pigeonpea. Theoretical and Applied Genetics, 130, 1773-1784. DOI 10.1007/s00122-017-2924-2