Portable Spectroscopy Calibration with Inexpensive and Simple Sampling Reference Alternatives for Dry Matter and Total Carotenoid Contents in Cassava Roots

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dc.contributor.authorAbincha, Wilfred
dc.contributor.authorIkeogu, Ugochukwu N.
dc.contributor.authorKawuki, Robert
dc.contributor.authorEgesi, Chiedozie
dc.contributor.authorRabbi, Ismail
dc.contributor.authorParkes, Elizabeth
dc.contributor.authorKulakow, Peter
dc.contributor.authorEdema, Richard
dc.contributor.authorGibson, Paul
dc.contributor.authorOwor, Betty-Elizabeth
dc.date.accessioned2022-06-12T18:59:37Z
dc.date.available2022-06-12T18:59:37Z
dc.date.issued2021
dc.description.abstractThe use of standard laboratory methods for trait evaluation is expensive and challenging, especially for low-resource breeding programs. For carotenoid assessment, rather than the standard HPLC method, these programs mostly rely on proxy approaches for quantitative total carotenoid content (TCC) assessment. To ensure data transferability and consistency, calibration models were developed using TCC iCheck and Chroma Meter proxy methods for the adoption of the alternative near-infrared phenotyping method in cassava. Calibration was developed for dry matter content (DMC) using a simple and inexpensive sampling procedure associated with the proxy TCC protocols. The partial least square (PLS) and random forest (RF) models were compared for the two traits, and the correlation (r) between the actual and predicted values in the training and validation (in bracket) sets of r = 0.85 (0.76) and r = 0.98 (0.82) with PLS and RF, respectively, for iCheck, and r = 0.99 (0.96) and r = 0.99 (0.96) with PLS and RF, respectively, for Chroma Meter, was obtained. The calibration result of r = 0.93 (0.83) and r = 0.99 (0.81) using PLS and RF, respectively, was obtained for DMC. This effort is valuable in carotenoids improvement and supports the ongoing effort in adopting portable spectrometers for rapid and cheap phenotyping in cassava.en_US
dc.identifier.citationAbincha,W.; Ikeogu, U.N.; Kawuki, R.; Egesi, C.; Rabbi, I.; Parkes, E.; Kulakow, P.; Edema, R.; Gibson, P.; Owor, B.-E. Portable Spectroscopy Calibration with Inexpensive and Simple Sampling Reference Alternatives for Dry Matter and Total Carotenoid Contents in Cassava Roots. Appl. Sci. 2021, 11, 1714. https://doi.org/10.3390/ app11041714en_US
dc.identifier.urihttps://doi.org/10.3390/ app11041714
dc.identifier.urihttps://nru.uncst.go.ug/handle/123456789/3932
dc.language.isoenen_US
dc.publisherApplied Sciencesen_US
dc.subjectCassavaen_US
dc.subjectnear-infrared spectroscopyen_US
dc.subjectCalibrationen_US
dc.subjectCarotenoidsen_US
dc.subjectiChecken_US
dc.subjectChroma Meteren_US
dc.titlePortable Spectroscopy Calibration with Inexpensive and Simple Sampling Reference Alternatives for Dry Matter and Total Carotenoid Contents in Cassava Rootsen_US
dc.typeArticleen_US
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