Portable Spectroscopy Calibration with Inexpensive and Simple Sampling Reference Alternatives for Dry Matter and Total Carotenoid Contents in Cassava Roots
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Date
2021
Journal Title
Journal ISSN
Volume Title
Publisher
Applied Sciences
Abstract
The use of standard laboratory methods for trait evaluation is expensive and challenging,
especially for low-resource breeding programs. For carotenoid assessment, rather than the standard
HPLC method, these programs mostly rely on proxy approaches for quantitative total carotenoid
content (TCC) assessment. To ensure data transferability and consistency, calibration models were
developed using TCC iCheck and Chroma Meter proxy methods for the adoption of the alternative
near-infrared phenotyping method in cassava. Calibration was developed for dry matter content
(DMC) using a simple and inexpensive sampling procedure associated with the proxy TCC protocols.
The partial least square (PLS) and random forest (RF) models were compared for the two traits, and
the correlation (r) between the actual and predicted values in the training and validation (in bracket)
sets of r = 0.85 (0.76) and r = 0.98 (0.82) with PLS and RF, respectively, for iCheck, and r = 0.99 (0.96)
and r = 0.99 (0.96) with PLS and RF, respectively, for Chroma Meter, was obtained. The calibration
result of r = 0.93 (0.83) and r = 0.99 (0.81) using PLS and RF, respectively, was obtained for DMC. This
effort is valuable in carotenoids improvement and supports the ongoing effort in adopting portable
spectrometers for rapid and cheap phenotyping in cassava.
Description
Keywords
Cassava, near-infrared spectroscopy, Calibration, Carotenoids, iCheck, Chroma Meter
Citation
Abincha,W.; Ikeogu, U.N.; Kawuki, R.; Egesi, C.; Rabbi, I.; Parkes, E.; Kulakow, P.; Edema, R.; Gibson, P.; Owor, B.-E. Portable Spectroscopy Calibration with Inexpensive and Simple Sampling Reference Alternatives for Dry Matter and Total Carotenoid Contents in Cassava Roots. Appl. Sci. 2021, 11, 1714. https://doi.org/10.3390/ app11041714