Phylogenic Analysis and Serotyping of Rice yellow mottle virus Strains in Uganda

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dc.contributor.authorUke, Ayaka
dc.contributor.authorAsea, Godfrey
dc.contributor.authorNatsuaki, Keiko T.
dc.date.accessioned2023-01-29T10:45:53Z
dc.date.available2023-01-29T10:45:53Z
dc.date.issued2016
dc.description.abstractt Rice yellow mottle virus(RYMV)is a majorrice (Oryza spp.) virus found only in Africa. In the present study, we collected samples of rice mainly from Eastern Uganda showing RYMV symptoms. The presence of RYMV was confirmed using ELISA and PCR. Some RYMV isolates in Uganda were sequenced and showed high homology levels with the Madagascar RYMV strain. They were grouped into S4ug strain in the East African lineage, showing high homology levels with isolates from neighboring countries. Serotyping based on the amino acid sequences of a RYMV coat protein gene fragment enabled to classify the Uganda isolates into Ser 4, in accordance with a previous serotyping report of other Ugandan isolates, using monoclonal antibodies. The present study revealed the widespread occurrence of RYMV in Uganda with a low diversity, irrespective of the origin of location and the rice varieties, as well as the first serotyping of Ugandan RYMV based on the amino acid sequences but not on monoclonal antibodies.en_US
dc.identifier.citationUKE, A., ASEA, G., & NATSUAKI, K. T. (2016). Phylogenic analysis and serotyping of Rice yellow mottle virus strains in Uganda. Tropical Agriculture and Development, 60(2), 81-88.en_US
dc.identifier.urihttps://www.jstage.jst.go.jp/article/jsta/60/2/60_81/_article/-char/ja/
dc.identifier.urihttps://nru.uncst.go.ug/handle/123456789/7379
dc.language.isoenen_US
dc.publisherTropical Agriculture and Developmenten_US
dc.subjectDetectionen_US
dc.subjectSequencingen_US
dc.subjectSobemovirusen_US
dc.subjectTransmissionen_US
dc.titlePhylogenic Analysis and Serotyping of Rice yellow mottle virus Strains in Ugandaen_US
dc.typeArticleen_US
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