Phylogenic Analysis and Serotyping of Rice yellow mottle virus Strains in Uganda
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Date
2016
Journal Title
Journal ISSN
Volume Title
Publisher
Tropical Agriculture and Development
Abstract
t Rice yellow mottle virus(RYMV)is a majorrice (Oryza spp.) virus found only in Africa. In the present study, we collected
samples of rice mainly from Eastern Uganda showing RYMV symptoms. The presence of RYMV was confirmed using ELISA and
PCR. Some RYMV isolates in Uganda were sequenced and showed high homology levels with the Madagascar RYMV strain.
They were grouped into S4ug strain in the East African lineage, showing high homology levels with isolates from neighboring
countries. Serotyping based on the amino acid sequences of a RYMV coat protein gene fragment enabled to classify the Uganda
isolates into Ser 4, in accordance with a previous serotyping report of other Ugandan isolates, using monoclonal antibodies. The
present study revealed the widespread occurrence of RYMV in Uganda with a low diversity, irrespective of the origin of location
and the rice varieties, as well as the first serotyping of Ugandan RYMV based on the amino acid sequences but not on monoclonal
antibodies.
Description
Keywords
Detection, Sequencing, Sobemovirus, Transmission
Citation
UKE, A., ASEA, G., & NATSUAKI, K. T. (2016). Phylogenic analysis and serotyping of Rice yellow mottle virus strains in Uganda. Tropical Agriculture and Development, 60(2), 81-88.