Elucidation of the Possible Mechanism of Analgesic Actions of Butanol Leaf Fraction of Olax subscorpioidea Oliv
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Date
2017
Journal Title
Journal ISSN
Volume Title
Publisher
Journal of Ethnopharmacology
Abstract
Ethnopharmacological relevance: Preparations of Olax subscorpioidea have been used
traditionally for the management of pains, inflammatory diseases, yellow fever, cancer
and rheumatism. Previously, the analgesic activity of its leaf extract have been reported.
Furthermore, an analgesic assay guided fractionation showed that the butanol soluble
fraction is the most active. However, the mechanism of this activity remains to be
elucidated. This present study investigated the possible pharmacological mechanisms involved in the analgesic activity of the butanol leaf fraction of Olax subscorpioidea
(BFOS) using the acetic acid induced writhing test in mice.
Materials and Methods: Animals were orally administered distilled water (10 ml/kg),
BFOS (1,000 mg/kg) and morphine (10 mg/kg) 60 minutes before i.p administration of
acetic acid and the resulting writhing were counted for 10 minutes. To establish the
possible mechanism(s) of action of BFOS, separate group of animals were pretreated
with naloxone (2 mg/kg, i.p), prazosin (1 mg/kg, i.p), yohimbine (1 mg/kg, i.p),
propranolol (20 mg/kg, i.p), metergoline (2 mg/kg, i.p), glibenclamide (5 mg/kg, i.p) and
l-arginine (50 mg/kg, i.p) 15 minutes before BFOS.
Results: BFOS and morphine showed marked analgesic activities (p<0.001); the
pretreatment of animals with naloxone, metergoline and l-arginine significantly (p<0.05
and p<0.001) reduced the analgesic activity of BFOS; however, pretreatment with
prazosin, yohimbine, propranolol and glinbenclamide showed no effect on its analgesic
0activity.
Conclusion: Results obtained in this study suggest the involvement of opioidergic,
serotonergic and nitric oxide-l-arginine pathways in the analgesic effect of butanol leaf
fraction of Olax subscorpioidea.
Description
Keywords
Olax subscorpioidea, Analgesic, Opioidergic, Serotonergic, Nitric-oxide and pain mechanism.
Citation
Citer Odoma, S., Zezi, A. U., Danjuma, N. M., Ahmed, A., & Magaji, M. G. (2017). Elucidation of the possible mechanism of analgesic actions of butanol leaf fraction of Olax subscorpioidea Oliv. Journal of ethnopharmacology, 199, 323-327.http://dx.doi.org/10.1016/j.jep.2016.12.052