RNAi-mediated resistance to Cassava brown streak Uganda virus in transgenic cassava

Simple item page
dc.contributor.authorYadav, Jitender S.
dc.contributor.authorOgwok, Emmanuel
dc.contributor.authorWagaba, Henry
dc.contributor.authorPatil, Basavaprabhu L.
dc.contributor.authorBagewadi, Basavaraj
dc.contributor.authorAlicai, Titus
dc.contributor.authorGaitan-Solis, Eliana
dc.contributor.authorTaylor, Nigel J.
dc.contributor.authorFauquet, Claude M.
dc.date.accessioned2022-06-25T17:56:19Z
dc.date.available2022-06-25T17:56:19Z
dc.date.issued2011
dc.description.abstractCassava brown streak disease (CBSD), caused by Cassava brown streak Uganda virus (CBSUV) and Cassava brown streak virus (CBSV), is of new epidemic importance to cassava (Manihot esculenta Crantz) production in East Africa, and an emerging threat to the crop in Central and West Africa. This study demonstrates that at least one of these two ipomoviruses, CBSUV, can be efficiently controlled using RNA interference (RNAi) technology in cassava. An RNAi construct targeting the near full-length coat protein (FL-CP) of CBSUV was expressed constitutively as a hairpin construct in cassava. Transgenic cassava lines expressing small interfering RNAs (siRNAs) against this sequence showed 100% resistance to CBSUV across replicated graft inoculation experiments. Reverse transcriptase-polymerase chain reaction (RT-PCR) analysis showed the presence of CBSUV in leaves and some tuberous roots from challenged controls, but not in the same tissues from transgenic plants. This is the first demonstration of RNAi-mediated resistance to the ipomovirus CBSUV in cassava.en_US
dc.identifier.citationYadav, J. S., Ogwok, E., Wagaba, H., Patil, B. L., Bagewadi, B., Alicai, T., ... & Fauquet, C. M. (2011). RNAi‐mediated resistance to Cassava brown streak Uganda virus in transgenic cassava. Molecular Plant Pathology, 12(7), 677-687. DOI: 10.1111/J.1364-3703.2010.00700.Xen_US
dc.identifier.other10.1111/J.1364-3703.2010.00700.X
dc.identifier.urihttps://nru.uncst.go.ug/handle/123456789/4085
dc.language.isoenen_US
dc.publisherMolecular Plant Pathologyen_US
dc.subjectRNAi-mediated resistanceen_US
dc.subjectCassava brown streaken_US
dc.subjectUgandaen_US
dc.subjectTransgenic cassavaen_US
dc.titleRNAi-mediated resistance to Cassava brown streak Uganda virus in transgenic cassavaen_US
dc.typeArticleen_US
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
RNAi-mediated resistance to Cassava brown streak Uganda virus.pdf
Size:
1.02 MB
Format:
Adobe Portable Document Format
Description:
Article
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Agricultural and Veterinary Sciences