RNAi-mediated resistance to Cassava brown streak Uganda virus in transgenic cassava
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Date
2011
Journal Title
Journal ISSN
Volume Title
Publisher
Molecular Plant Pathology
Abstract
Cassava brown streak disease (CBSD), caused by Cassava brown
streak Uganda virus (CBSUV) and Cassava brown streak virus
(CBSV), is of new epidemic importance to cassava (Manihot
esculenta Crantz) production in East Africa, and an emerging
threat to the crop in Central and West Africa. This study demonstrates
that at least one of these two ipomoviruses, CBSUV, can
be efficiently controlled using RNA interference (RNAi) technology
in cassava. An RNAi construct targeting the near full-length
coat protein (FL-CP) of CBSUV was expressed constitutively as a
hairpin construct in cassava. Transgenic cassava lines expressing
small interfering RNAs (siRNAs) against this sequence showed
100% resistance to CBSUV across replicated graft inoculation
experiments. Reverse transcriptase-polymerase chain reaction
(RT-PCR) analysis showed the presence of CBSUV in leaves and
some tuberous roots from challenged controls, but not in the
same tissues from transgenic plants. This is the first demonstration
of RNAi-mediated resistance to the ipomovirus CBSUV in
cassava.
Description
Keywords
RNAi-mediated resistance, Cassava brown streak, Uganda, Transgenic cassava
Citation
Yadav, J. S., Ogwok, E., Wagaba, H., Patil, B. L., Bagewadi, B., Alicai, T., ... & Fauquet, C. M. (2011). RNAi‐mediated resistance to Cassava brown streak Uganda virus in transgenic cassava. Molecular Plant Pathology, 12(7), 677-687. DOI: 10.1111/J.1364-3703.2010.00700.X