Shoot organogenesis from leaf discs of the African ginger (Mondia whitei (Hook.f.) Skeels), an endangered medicinal plant

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dc.contributor.authorPatricia, Driciru
dc.contributor.authorStephen, Buah
dc.contributor.authorJohn, Adriko
dc.date.accessioned2022-02-04T12:21:08Z
dc.date.available2022-02-04T12:21:08Z
dc.date.issued2021
dc.description.abstractThe African ginger (Mondia whitei) is an endangered, endemic African medicinal plant, widely used as an aphrodisiac and to manage gastrointestinal problems and anorexia. Due to high demand, it is over-utilized from the wild without replenishment and destroyed by inappropriate harvesting methods. Therefore, there is a need to develop an efficient conservation system for the African ginger. This study focused on developing an in vitro propagation procedure for the African ginger through indirect shoot organogenesis. Optimal callus formation was induced from leaf explants cultured on Murashige and Skoog (MS) basal medium fortified with 1.5 mg L−1 2,4-dichlorophenoxyacetic acid (2,4-D). The 1.5 mg L−1 6-benzylaminopurine (BAP) concentration was optimal with 50% of the callus-forming buds. For shoot formation, media with 2.0 mg L−1 BAP and 1.0 mg L−1 NAA resulted in 60% of the buds developing shoots. Regenerated shoots were rooted on woody plant medium (WPM) fortified with 0.1 mg L−1 BAP and no rooting hormone included. The survival rate of weaned and acclimatized plants was 70% and up to 80% respectively. The study demonstrates an in vitro propagation route for the African gingerwhich could support itsmass production for medicinal use, availing of planting materials, conservation, and commercialization purposes.en_US
dc.identifier.citationPatricia, D., Stephen, B., & John, A. (2021). Shoot organogenesis from leaf discs of the African ginger (Mondia whitei (Hook. f.) Skeels), an endangered medicinal plant. In Vitro Cellular & Developmental Biology-Plant, 57(3), 493-498. https://doi.org/10.1007/s11627-020-10146-0en_US
dc.identifier.urihttps://doi.org/10.1007/s11627-020-10146-0
dc.identifier.urihttps://nru.uncst.go.ug/xmlui/handle/123456789/1887
dc.language.isoenen_US
dc.publisherIn Vitro Cellular & Developmental Biology-Planten_US
dc.subjectRegenerationen_US
dc.subjectIn vitro propagationen_US
dc.subjectConservationen_US
dc.subjectOrganogenesisen_US
dc.titleShoot organogenesis from leaf discs of the African ginger (Mondia whitei (Hook.f.) Skeels), an endangered medicinal planten_US
dc.typeArticleen_US
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