Shoot organogenesis from leaf discs of the African ginger (Mondia whitei (Hook.f.) Skeels), an endangered medicinal plant
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Date
2021
Journal Title
Journal ISSN
Volume Title
Publisher
In Vitro Cellular & Developmental Biology-Plant
Abstract
The African ginger (Mondia whitei) is an endangered, endemic African medicinal plant, widely used as an aphrodisiac and to
manage gastrointestinal problems and anorexia. Due to high demand, it is over-utilized from the wild without replenishment and
destroyed by inappropriate harvesting methods. Therefore, there is a need to develop an efficient conservation system for the
African ginger. This study focused on developing an in vitro propagation procedure for the African ginger through indirect shoot
organogenesis. Optimal callus formation was induced from leaf explants cultured on Murashige and Skoog (MS) basal medium
fortified with 1.5 mg L−1 2,4-dichlorophenoxyacetic acid (2,4-D). The 1.5 mg L−1 6-benzylaminopurine (BAP) concentration
was optimal with 50% of the callus-forming buds. For shoot formation, media with 2.0 mg L−1 BAP and 1.0 mg L−1 NAA
resulted in 60% of the buds developing shoots. Regenerated shoots were rooted on woody plant medium (WPM) fortified with
0.1 mg L−1 BAP and no rooting hormone included. The survival rate of weaned and acclimatized plants was 70% and up to 80%
respectively. The study demonstrates an in vitro propagation route for the African gingerwhich could support itsmass production
for medicinal use, availing of planting materials, conservation, and commercialization purposes.
Description
Keywords
Regeneration, In vitro propagation, Conservation, Organogenesis
Citation
Patricia, D., Stephen, B., & John, A. (2021). Shoot organogenesis from leaf discs of the African ginger (Mondia whitei (Hook. f.) Skeels), an endangered medicinal plant. In Vitro Cellular & Developmental Biology-Plant, 57(3), 493-498. https://doi.org/10.1007/s11627-020-10146-0