Agaricus Subrufescens Ameliorates Ovarian Dysfunction and Regulates Altered Biochemical Parameters in Rats with Letrozole Induced Polycystic Ovarian Syndrome
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Date
2020
Journal Title
Journal ISSN
Volume Title
Publisher
Journal of Phytomedicine and Therapeutics
Abstract
To examine the impact of an ethonolic extract of Agaricus subrufescens on rat models of Polycystic
Ovarian Syndrome (PCOS) produced by letrozole.
Methods
Thirty female Wistar rats were split into five six-rat groups. Negative controls received 1 mL of 0.5%
carboxy methylcellulose (CMC). Other groups received letrozole (1 mg/kg) for 21 days to induce PCOS.
Positive control animals were slaughtered on day 22. The test and standard groups received treatment
from the 22nd to the 36th day. The test group received Agaricus subrufescens ethanoic extract (200
mg/kg, 400 mg/kg, p.o.) and the standard group clomiphene citrate (1 mg/kg). Estimated blood glucose,
total cholesterol, triglycerides, and hormonal changes like increased testosterone, oestrogen, and
decreased progesterone with menstrual irregularity confirmed by vaginal smears and histopathological
ovary changes in polycystic ovarian disease control.
Results
Agaricus subrufescens reduced blood glucose, testosterone, anovulation, and menstrual irregularity. All
therapies markedly corrected SGOT and SGPT levels. Letrozole-induced PCOS rats had elevated urea and
creatinine. This study suggests that Agaricus subrufescens therapy protects renal function by lowering
serum urea and creatinine. In letrozole-induced PCOS rats, suppressing hepatic synthesis, increasing
ovarian follicle immaturity, and boosting androgen discharges increase liver and ovary weight. Endocrine
organ weight decreased in all treatment groups. PCOS control histopathology indicated more cysts and
theca lutein cells. PCOS control rats had more cysts than treatment groups.
Conclusion
Oral letrozole produced polycystic ovarian disease in this research. It showed elevated blood glucose,
total cholesterol, triglycerides, and hormonal changes like increased testosterone and oestrogen and
decreased progesterone with menstrual irregularity confirmed by vaginal smears and histopathological
changes in the ovary of polycystic ovarian disease control. Blood glucose, total cholesterol, and
testosterone levels dropped in Agaricus subrufescens treatment groups.
Description
Keywords
Agaricus Subrufescens, Polycystic ovarian syndrome, Letrazole, Histopathological study, Vaginal smear, Estrogen, Blood glucose level
Citation
Saidi, O., Umar, Z. A., Muhammed, D. N., Abubakar, A., Magaji, M. G., & Sani, M. A. (2020). Preliminary Evaluation of the Acute and Sub-Acute Anti-Inflammatory Activities of Aqueous and Butanol Leaf Fractions of Olax subscorpioidea Oliv.(Olacaceae). Journal of Phytomedicine and Therapeutics, 19(2), 398-409. https://doi.org/10.21203/rs.3.rs-2889034/v1