Coreceptor Tropism in Human Immunodeficiency Virus Type 1 Subtype D: High Prevalence of CXCR4 Tropism and Heterogeneous Composition of Viral Populations

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dc.contributor.authorHuang, Wei
dc.contributor.authorEshleman, Susan H.
dc.contributor.authorToma, Jonathan
dc.contributor.authorFransen, Signe
dc.contributor.authorStawiski, Eric
dc.contributor.authorPaxinos, Ellen E.
dc.contributor.authorWhitcomb, Jeannette M.
dc.contributor.authorYoung, Alicia M.
dc.contributor.authorDonnell, Deborah
dc.contributor.authorMmiro, Francis
dc.contributor.authorMusoke, Philippa
dc.contributor.authorGuay, Laura A.
dc.contributor.authorJackson, Brooks
dc.contributor.authorParkin, Neil T.
dc.contributor.authorPetropoulos, Christos J.
dc.date.accessioned2021-12-15T13:03:56Z
dc.date.available2021-12-15T13:03:56Z
dc.date.issued2007
dc.description.abstractIn human immunodeficiency virus type 1 (HIV-1) subtype B, CXCR4 coreceptor use ranges from 20% in early infection to 50% in advanced disease. Coreceptor use by non-subtype B HIV is less well characterized. We studied coreceptor tropism of subtype A and D HIV-1 collected from 68 pregnant, antiretroviral drug-naive Ugandan women (HIVNET 012 trial). None of 33 subtype A or 10 A/D-recombinant viruses used the CXCR4 coreceptor. In contrast, nine (36%) of 25 subtype D viruses used both CXCR4 and CCR5 coreceptors. Clonal analyses of the nine subtype D samples with dual or mixed tropism revealed heterogeneous viral populations comprised of X4-, R5-, and dual-tropic HIV-1 variants. In five of the six samples with dual-tropic strains, V3 loop sequences of dual-tropic clones were identical to those of cocirculating R5-tropic clones, indicating the presence of CXCR4 tropism determinants outside of the V3 loop. These dual-tropic variants with R5-tropic-like V3 loops, which we designated “dual-R,” use CCR5 much more efficiently than CXCR4, in contrast to dual-tropic clones with X4-tropic-like V3 loops (“dual-X”). These observations have implications for pathogenesis and treatment of subtype D-infected individuals, for the association between V3 sequence and coreceptor tropism phenotype, and for understanding potential mechanisms of evolution from exclusive CCR5 use to efficient CXCR4 use by subtype D HIV-1.en_US
dc.identifier.citationHuang, W., Eshleman, S. H., Toma, J., Fransen, S., Stawiski, E., Paxinos, E. E., ... & Petropoulos, C. J. (2007). Coreceptor tropism in human immunodeficiency virus type 1 subtype D: high prevalence of CXCR4 tropism and heterogeneous composition of viral populations. Journal of virology, 81(15), 7885-7893.doi:10.1128/JVI.00218-07en_US
dc.identifier.other10.1128/JVI.00218-07
dc.identifier.urihttps://nru.uncst.go.ug/xmlui/handle/123456789/619
dc.language.isoenen_US
dc.publisherJournal of virologyen_US
dc.subjectCoreceptor Tropismen_US
dc.subjectHuman Immunodeficiency Virusen_US
dc.subjectCXCR4 Tropismen_US
dc.subjectHeterogeneous Compositionen_US
dc.subjectViral Populationsen_US
dc.titleCoreceptor Tropism in Human Immunodeficiency Virus Type 1 Subtype D: High Prevalence of CXCR4 Tropism and Heterogeneous Composition of Viral Populationsen_US
dc.typeArticleen_US
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