The Effect of Antiretroviral Therapy Initiation on the Vaginal Microbiome in HIV-Infected Women

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Liu, Cindy M.
Packman, Zoe R.
Abraham, Alison G.
Serwadda, David M.
Nalugoda, Fred
Aziz, Maliha
Prodger, Jessica L.
Kaul, Rupert
Kalibbala, Sarah
Gray, Ronald H.
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The Journal of infectious diseases
The impact of antiretroviral therapy (ART) initiation on the vaginal microbiome is unknown. This is of particular importance among women living in sub-Saharan Africa. Understanding this relationship could help elucidate if and how the host immune system interacts with the vaginal microbiome. Methods. The vaginal microbiome of HIV-1/HSV-2-coinfected women (n = 92) in Uganda was evaluated from self-collected vaginal swabs 1 month pre-ART and at 4 and 6 months post–ART initiation. The vaginal microbiome was characterized by 16S rRNA genebased sequencing and quantitative polymerase chain reaction. Vaginal community state types (CSTs) were identified using proportional abundance data. Changes in microbiome composition were assessed with permutational analyses of variance (PerMANOVA).
Antiretroviral therapy, Anaerobes, HIV, Microbiome, Vaginal dysbiosis.
Grabowski, M. K., Patel, E. U., Nakigozi, G., Ssempijja, V., Ssekubugu, R., Ssekasanvu, J., ... & Reynolds, S. J. (2021). Prevalence and Predictors of Persistent Human Immunodeficiency Virus Viremia and Viral Rebound After Universal Test and Treat: A Population-Based Study. The Journal of infectious diseases, 223(7), 1150-1160. DOI: 10.1093/ofid/ofz328