The prevalence and genetic characterisation of Cryptosporidium isolates from cattle in Kiruhura district, South Western Uganda

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dc.contributor.authorWitto, Sarah Gift
dc.contributor.authorKankya, Clovice
dc.contributor.authorAkurut, Gloria
dc.contributor.authorMugasa, Claire Mack
dc.contributor.authorKazibwe, Anne
dc.contributor.authorOchwo, Sylvester
dc.date.accessioned2022-08-18T20:37:05Z
dc.date.available2022-08-18T20:37:05Z
dc.date.issued2021
dc.description.abstractCryptosporidium is an emerging opportunistic zoonotic pathogen that causes diarrheal illness in a wide range of hosts including livestock and humans. This study set out to establish the prevalence of Cryptosporidium as well as the circulating genotypes in order to elucidate the potential role of cattle in the spread of human cryptosporidiosis. Rectal coprological samples from 363 cattle in 11 households in Kiruhura district, Southwestern Uganda were collected and screened for the presence of Cryptosporidium oocysts using the phenol auramine staining method followed by fluorescent microscopy. DNA was extracted from the microscopy positive samples and the COWP gene amplified using PCR. PCR products were sequenced and subjected to phylogenetic analysis. Additionally a multiplex realtime PCR was used to identify the Cryptosporidium spp. Multivariable mixed effect logistic regression models were used to identify potential risk factors for Cryptosporidium infection. The overallen_US
dc.identifier.citationWitto, S. G., Kankya, C., Akurut, G., Mugasa, C. M., Kazibwe, A., & Ochwo, S. (2021). The prevalence and genetic characterisation of Cryptosporidium isolates from cattle in Kiruhura district, South Western Uganda. Journal of Parasitic Diseases, 45(3), 778-789. https://doi.org/10.1007/s12639-021-01361-6en_US
dc.identifier.urihttps://doi.org/10.1007/s12639-021-01361-6
dc.identifier.urihttps://nru.uncst.go.ug/handle/123456789/4312
dc.language.isoenen_US
dc.publisherJournal of Parasitic Diseasesen_US
dc.subjectCryptosporidiumen_US
dc.subjectGenotypingen_US
dc.subjectC. hominisen_US
dc.subjectUgandaen_US
dc.titleThe prevalence and genetic characterisation of Cryptosporidium isolates from cattle in Kiruhura district, South Western Ugandaen_US
dc.typeArticleen_US
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