The prevalence and genetic characterisation of Cryptosporidium isolates from cattle in Kiruhura district, South Western Uganda
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Date
2021
Journal Title
Journal ISSN
Volume Title
Publisher
Journal of Parasitic Diseases
Abstract
Cryptosporidium is an emerging opportunistic
zoonotic pathogen that causes diarrheal illness in a wide
range of hosts including livestock and humans. This study
set out to establish the prevalence of Cryptosporidium as
well as the circulating genotypes in order to elucidate the
potential role of cattle in the spread of human cryptosporidiosis.
Rectal coprological samples from 363 cattle
in 11 households in Kiruhura district, Southwestern
Uganda were collected and screened for the presence of
Cryptosporidium oocysts using the phenol auramine
staining method followed by fluorescent microscopy. DNA
was extracted from the microscopy positive samples and
the COWP gene amplified using PCR. PCR products were
sequenced and subjected to phylogenetic analysis. Additionally
a multiplex realtime PCR was used to identify the
Cryptosporidium spp. Multivariable mixed effect logistic
regression models were used to identify potential risk
factors for Cryptosporidium infection. The overall
Description
Keywords
Cryptosporidium, Genotyping, C. hominis, Uganda
Citation
Witto, S. G., Kankya, C., Akurut, G., Mugasa, C. M., Kazibwe, A., & Ochwo, S. (2021). The prevalence and genetic characterisation of Cryptosporidium isolates from cattle in Kiruhura district, South Western Uganda. Journal of Parasitic Diseases, 45(3), 778-789. https://doi.org/10.1007/s12639-021-01361-6