Characterization of Foot-And-Mouth Disease Viruses (FMDVs) from Ugandan Cattle Outbreaks during 2012-2013: Evidence for Circulation of Multiple Serotypes

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dc.contributor.authorNamatovu, Alice
dc.contributor.authorTjørnehøj, Kirsten
dc.contributor.authorBelsham, Graham J.
dc.contributor.authorDhikusooka, Moses T.
dc.contributor.authorWekesa, Sabenzia N.
dc.contributor.authorMuwanika, Vincent B.
dc.contributor.authorSiegismund, Hans R.
dc.contributor.authorAyebazibwe, Chrisostom
dc.date.accessioned2023-01-24T16:45:52Z
dc.date.available2023-01-24T16:45:52Z
dc.date.issued2015
dc.description.abstractTo investigate the foot-and-mouth disease virus (FMDV) serotypes circulating in Uganda’s cattle population, both serological and virological analyses of samples from outbreaks that occurred during 2012–2013 were performed. Altogether, 79 sera and 60 oropharyngeal fluid (OP)/ tissue/oral swab samples were collected from herds with reported FMD outbreaks in seven different Ugandan districts. Overall, 61/79 (77%) of the cattle sera were positive for antibodies against FMDV by PrioCHECK FMDV NS ELISA and solid phase blocking ELISA detected titres 80 for serotypes O, SAT 1, SAT 2 and SAT 3 in 41, 45, 30 and 45 of these 61 seropositive samples, respectively. Virus neutralisation tests detected the highest levels of neutralising antibodies (titres 45) against serotype O in the herds from Kween and Rakai districts, against SAT 1 in the herd from Nwoya district and against SAT 2 in the herds fromKiruhura, Isingiro and Ntungamo districts. The isolation of a SAT 2 FMDV from Isingiro was consistent with the detection of high levels of neutralising antibodies against SAT 2; sequencing (for the VP1 coding region) indicated that this virus belonged to lineage I within this serotype, like the currently used vaccine strain. From theWakiso district 11 tissue/swab samples were collected; serotype A FMDV, genotype Africa (G-I), was isolated from the epithelial samples. This study shows that within a period of less than one year, FMD outbreaks in Uganda were caused by four different serotypes namely O, A, SAT 1 and SAT 2. Therefore, to enhance the control of FMD in Uganda, there is need for efficient and timely determination of outbreak virus strains/serotypes and vaccine matching. The value of incorporating serotype A antigen into the imported vaccines along with the current serotype O, SAT 1 and SAT 2 strains should be considered.en_US
dc.identifier.citationNamatovu A, Tjørnehøj K, Belsham GJ, Dhikusooka MT, Wekesa SN, Muwanika VB, et al. (2015) Characterization of Foot-And-Mouth Disease Viruses (FMDVs) from Ugandan Cattle Outbreaks during 2012-2013: Evidence for Circulation of Multiple Serotypes. PLoS ONE 10(2): e0114811. doi:10.1371/ journal.pone.0114811en_US
dc.identifier.other10.1371/ journal.pone.0114811
dc.identifier.urihttps://nru.uncst.go.ug/handle/123456789/7165
dc.language.isoenen_US
dc.publisherPLoS Oneen_US
dc.subjectFoot-And-Mouth Disease Viruses (FMDVs)en_US
dc.subjectCattleen_US
dc.subjectSerotypesen_US
dc.titleCharacterization of Foot-And-Mouth Disease Viruses (FMDVs) from Ugandan Cattle Outbreaks during 2012-2013: Evidence for Circulation of Multiple Serotypesen_US
dc.typeArticleen_US
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