Characterization of Foot-And-Mouth Disease Viruses (FMDVs) from Ugandan Cattle Outbreaks during 2012-2013: Evidence for Circulation of Multiple Serotypes
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Date
2015
Journal Title
Journal ISSN
Volume Title
Publisher
PLoS One
Abstract
To investigate the foot-and-mouth disease virus (FMDV) serotypes circulating in Uganda’s cattle
population, both serological and virological analyses of samples from outbreaks that occurred
during 2012–2013 were performed. Altogether, 79 sera and 60 oropharyngeal fluid (OP)/
tissue/oral swab samples were collected from herds with reported FMD outbreaks in seven different
Ugandan districts. Overall, 61/79 (77%) of the cattle sera were positive for antibodies
against FMDV by PrioCHECK FMDV NS ELISA and solid phase blocking ELISA detected titres
80 for serotypes O, SAT 1, SAT 2 and SAT 3 in 41, 45, 30 and 45 of these 61 seropositive
samples, respectively. Virus neutralisation tests detected the highest levels of neutralising antibodies
(titres 45) against serotype O in the herds from Kween and Rakai districts, against
SAT 1 in the herd from Nwoya district and against SAT 2 in the herds fromKiruhura, Isingiro
and Ntungamo districts. The isolation of a SAT 2 FMDV from Isingiro was consistent with the
detection of high levels of neutralising antibodies against SAT 2; sequencing (for the VP1 coding
region) indicated that this virus belonged to lineage I within this serotype, like the currently
used vaccine strain. From theWakiso district 11 tissue/swab samples were collected; serotype
A FMDV, genotype Africa (G-I), was isolated from the epithelial samples. This study shows that
within a period of less than one year, FMD outbreaks in Uganda were caused by four different
serotypes namely O, A, SAT 1 and SAT 2. Therefore, to enhance the control of FMD in Uganda,
there is need for efficient and timely determination of outbreak virus strains/serotypes and vaccine
matching. The value of incorporating serotype A antigen into the imported vaccines along
with the current serotype O, SAT 1 and SAT 2 strains should be considered.
Description
Keywords
Foot-And-Mouth Disease Viruses (FMDVs), Cattle, Serotypes
Citation
Namatovu A, Tjørnehøj K, Belsham GJ, Dhikusooka MT, Wekesa SN, Muwanika VB, et al. (2015) Characterization of Foot-And-Mouth Disease Viruses (FMDVs) from Ugandan Cattle Outbreaks during 2012-2013: Evidence for Circulation of Multiple Serotypes. PLoS ONE 10(2): e0114811. doi:10.1371/ journal.pone.0114811