Anti-nociceptive, anti-inflammatory and possible mechanism of antinociceptive action of methanol leaf extract of Nymphaea lotus Linn (Nymphaeceae)
Loading...
Date
2021
Journal Title
Journal ISSN
Volume Title
Publisher
Journal of Traditional and Complementary Medicine
Abstract
The leaf of Nymphaea lotus has been used traditionally for the management of pain and inflammatory
diseases. The methanol leaf extract of Nymphaea lotus (NLE) was evaluated for possible anti-nociceptive
and anti-inflammatory activities in rats and mice (at the doses of 250, 500 and 1,000 mg/kg) to investigate
the existence of scientific basis for the folkloric use of the plant. The standard drugs used were
piroxicam (10 mg/kg) and morphine (10 mg/kg). The possible pharmacological mechanism involved in
the anti-nociceptive activity was also investigated. The acute toxicity was determined in mice and rats
using method of Lorke. The anti-nociceptive activity was evaluated using acetic acid-induced writhing
and hot plate tests in mice, while the anti-inflammatory activity was evaluated using carrageenaninduced
hind paw edema model in rats. The oral median lethal dose of NLE was found to be greater
than 5,000 mg/kg in rats and mice. NLE demonstrated significant and dose-dependent protection against
acetic acid induced writhes and increased the reaction time of mice in hot plate test. Pretreatment of the
animals with naloxone (2 mg/kg) significantly (p < 0.05) attenuated the anti-nociception elicited by both
NLE and morphine. NLE at the doses of 250 and 1,000 mg/kg significantly (p < 0.05) decreased rat paw
edema at the 2nd hour in the carrageenan-induced paw edema test. The result of the study revealed that
Nymphaea lotus possesses anti-nociceptive activities which may be mediated via the opioidergic system
as well as mild anti-inflammatory activities thus providing scientific basis for the use of the plant in the
management of pain and inflammatory diseases.
Description
Keywords
Acetic acid, Analgesia, Anti-Inflammation, Nymphaea lotus, Hot plate test, Opioidergic
Citation
Rege, M. G., Ayanwuyi, L. O., Zezi, A. U., & Odoma, S. (2021). Anti-nociceptive, anti-inflammatory and possible mechanism of anti-nociceptive action of methanol leaf extract of Nymphaea lotus Linn (Nymphaeceae). Journal of Traditional and Complementary Medicine, 11(2), 123-129. https://doi.org/10.1016/j.jtcme.2020.02.010