Hydration dependent dynamics in RNA
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Date
2009
Journal Title
Journal ISSN
Volume Title
Publisher
Journal of biomolecular NMR
Abstract
The essential role played by local and collective
motions in RNA function has led to a growing interest
in the characterization of RNA dynamics. Recent investigations
have revealed that even relatively simple RNAs
experience complex motions over multiple time scales
covering the entire ms–ps motional range. In this work, we
use deuterium solid-state NMR to systematically investigate
motions in HIV-1 TAR RNA as a function of hydration.
We probe dynamics at three uridine residues in
different structural environments ranging from helical to
completely unrestrained. We observe distinct and substantial
changes in 2H solid-state relaxation times and
lineshapes at each site as hydration levels increase. By
comparing solid-state and solution state 13C relaxation
measurements, we establish that ns–ls motions that may be
indicative of collective dynamics suddenly arise in the
RNA as hydration reaches a critical point coincident with
the onset of bulk hydration. Beyond that point, we observe
smaller changes in relaxation rates and lineshapes in these
highly hydrated solid samples, compared to the dramatic
activation of motion occurring at moderate hydration.
Description
Keywords
RNA, Dynamics, Hydration, Solid state NMR, Deuterium NMR, Relaxation
Citation
Olsen, G. L., Bardaro, M. F., Echodu, D. C., Drobny, G. P., & Varani, G. (2009). Hydration dependent dynamics in RNA. Journal of biomolecular NMR, 45(1), 133-142. DOI 10.1007/s10858-009-9355-6