B-cell non-Hodgkin lymphomas in Uganda: an immunohistochemical appraisal on tissue microarray
Loading...
Date
2008
Journal Title
Journal ISSN
Volume Title
Publisher
Human pathology
Abstract
The most common non-Hodgkin lymphomas in Uganda are neoplasms of B-cell derivation.
The field of B-cell lymphoma immunophenotype has rapidly progressed because of the increasing
availability of markers applicable to routine sections. Although the latter have allowed the identification
of distinctive lymphoma entities in the developed countries, such approach has not yet been used in
Uganda. One hundred twenty-nine formalin-fixed, paraffin-embedded tissue samples from the
Department of Pathology of Makerere University were used for tissue micro-array (TMA) construction.
Four-micrometer-thick sections were cut from TMAs and stained with hematoxylin and eosin and
Giemsa. Theywere also used for immunohistochemistry and in situ hybridization.According to morphology
and immunohistochemistry, lymphoid neoplasms were classified as Burkitt's lymphoma (BL) (95 cases),
diffuse large B-cell lymphoma (19 cases), mantle cell lymphoma (4 cases), and B-cell lymphoblastic
lymphoma (1 case). In BL, a homogeneous phenotype (CD10+, Bcl-6+, Bcl-2- , MUM1/IRF4-, and Ki-67
~100%) and a stable Epstein-Barr virus integration were found. A distinctive and unusual feature was the
frequent plasma cellular differentiation, along with the positivity for CD30 and CD138 (recorded in 35 and
43 cases, respectively). According to our findings, most non-Hodgkin B-cell tumors in Uganda are endemic
BLs followed by diffuse large B-cell lymphomas. The rest consist of rare but clinically important entities
such as mantle cell lymphoma and B-cell lymphoblastic lymphoma. The availability of TMAs and
immunohistochemistry has enabled us to precisely categorize tumors that have so far been diagnosed in
Uganda as “high-grade/aggressive” lymphomas on the basis of cell morphology alone.
© 2008 Elsevier Inc. All rights reserved.
Description
Keywords
Non-Hodgkin lymphoma, Burkitt lymphoma, Phenotype, Uganda, Tissue microarray
Citation
Tumwine, L. K., Campidelli, C., Righi, S., Neda, S., Byarugaba, W., & Pileri, S. A. (2008). B-cell non-Hodgkin lymphomas in Uganda: an immunohistochemical appraisal on tissue microarray. Human pathology, 39(6), 817-823. doi:10.1016/j.humpath.2007.10.025