Banana influences on differential expression of hypersensitive response and pathogenicity gene f (hrpf) in Xanthomonas campestris pv. musacearum
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Date
2017
Journal Title
Journal ISSN
Volume Title
Publisher
African Journal of Biotechnology
Abstract
Banana Xanthomonas wilt is a devastating disease of cultivated banana in East and Central Africa,
manifesting as initial leaf wilting, premature fruit ripening and eventual death of all infected plants
leading to total yield loss. In order to contribute towards development of effective disease control
options, hrpf expression by Xanthomonas campestris pv musacearum (Xcm) during establishment of
Xanthomonas wilt infection was determined. To successfully initiate infection, plant pathogenic
bacteria deliver effector proteins into host cells using specialized protein transport system such as the
Type III secretion system (TTSS). It is a syringe needle-like translocation apparatus essential for
delivery of effector proteins into the host cells and hypersensitive response and pathogenicity gene f
(hrpf) encodes one of the structural proteins for effector protein delivery. In this study, hrpf expression
by Xcm during establishment of Xanthomonas wilt infection was determined in minimal medium
amended with extracts from susceptible banana genotypes and banana host plants. Total RNA was
isolated from Xcm recovered from inoculated plants and also from minimal medium amended with
banana extracts; cDNA synthesised and hrpf amplified by PCR using gene specific primers. Findings
showed that Xcm multiplied in susceptible host banana and minimal medium amended with their
extracts but not in resistant M. balbisiana and its extract. hrpf gene was thus amplified from cDNA
samples of susceptible banana genotypes and their extracts suggesting its expression and involvement
in the successful establishment of Xanthomonas wilt disease by Xcm.
Description
Keywords
Xanthomonas campestris pv. musacearum (Xcm), Type three secretion system (TTSS), Hypersensitive response and pathogenicityf (hrpf) gene, Banana
Citation
Mudonyi, B., Tusiime, G., Kubiriba, J., Tushemereirwe, W. K., & Changa, C. M. (2017). Banana influences on differential expression of hypersensitive response and pathogenicity gene f (hrpf) in Xanthomonas campestris pv. musacearum. African Journal of Biotechnology, 16(10), 463-469. DOI: 10.5897/AJB2016.15659