Distribution and transmission of Mycobacterium tuberculosis complex lineages among children in peri-urban Kampala, Uganda
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Date
2015
Journal Title
Journal ISSN
Volume Title
Publisher
BMC pediatrics
Abstract
To gain insight into the transmission of tuberculosis (TB) in peri-urban Kampala-Uganda, we performed a
household contact study using children as a surrogate for recent transmission of Mycobacterium tuberculosis (MTB).
Using this approach, we sought to understand M. tuberculosis complex (MTBC) lineage diversity, distribution and how
these relate to TB transmission to exposed children.
Method: MTBC isolates from children aged ≤ 15 years, collected from 2002 to 2010 in a household-contact study, were
analyzed using a LightCycler RT-PCR SNP genotyping assay (LRPS). The resultant genotypic data was used to determine
associations between MTBC lineage and the children’s clinical and epidemiological characteristics.
Results and discussion: Of the 761 children surveyed, 9 % (69/761) had culture-positive TB an estimate in the
range of global childhood TB; of these 71 % (49/69) were infected with an MTBC strain of the “Uganda family”,
17 % (12/69) infected with MTBC lineage 4 strains other than MTBC Uganda family and 12 % (8/69) infected with
MTBC lineage 3, thereby disproportionately causing TB in the study area. Overall the data showed no correlation
between the MTBC lineages studied and transmission (OR = 0.304; P-value = 0.251; CI: 95 %; 0.039-2.326) using
children a proxy for TB transmission.
Conclusions: Our findings indicate that MTBC Uganda family strains are the main cause of TB in children in peri-urban
Kampala. Furthermore, MTBC lineages did not differ in their transmissibility to children.
Description
Keywords
Household, Childhood tuberculosis, Transmission, MTBC lineages
Citation
Wampande, E. M., Mupere, E., Jaganath, D., Nsereko, M., Mayanja, H. K., Eisenach, K., ... & Joloba, M. L. (2015). Distribution and transmission of Mycobacterium tuberculosis complex lineages among children in peri-urban Kampala, Uganda. BMC pediatrics, 15(1), 1-7. DOI 10.1186/s12887-015-0455-z