Browsing by Author "Nasinyama, George William"
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Item Brucellosis: Community, Medical and Veterinary Workers’ Knowledge, Attitudes, and Practices in Northern Uganda(Int J One Health, 2017) Nabirye, Harriet Muloki; Erume, Joseph; Nasinyama, George William; Kungu, Joseph Morison; Nakavuma, Jesca; Ongeng, Duncan; Owiny, David OkelloThis study aimed at determining the knowledge, attitudes, and practices of the community, medical and veterinary workers regarding brucellosis.A cross-sectional study was conducted at selected health facilities in Apac, Gulu, Lira, and Pader districts of Northern Uganda using a standardized questionnaire. A total of 251 patients testing positive for brucellosis using the Brucella plate agglutination test, 59 medical and 29 veterinary workers were studied. Chi-square test at 95% confidence level was used to analyze data.Only 8% patients, 15.3% medical, and 21.4% veterinary workers were knowledgeable on transmission methods and symptoms for brucellosis and knowledge differed according to the level of education among patients (p=0.001), medical (p=0.001), and veterinary workers (p=0.012). Over 80% patients, medical and veterinary workers had a positive attitude. Only 8% patients, 13.6% medical, and 7.1% veterinary workers had good practices regarding brucellosis control. Poor knowledge, poor practices, and positive attitude provide an opportunity for health education and policy formulation for the control of brucellosis. The prevalence studies of human and animal brucellosis are recommended to determine the magnitude of the problem.Item Diatomaceous Earth Usage in The Agriculture Sector in Uganda And Its Characterization: Current Status and Anticipated Developments(Journal of Agricultural Science and Food Technology, 2019) Isabirye, Robert Alex; Waiswa, Charles; Kabi, Fred; Nanyeenya, William N.; Biryomumaisho, Savino; Acai-Okwee, James; Okello, Samuel; Omonuk Akello, Beatrice; Mwesigwa, Moses; Lumu, Richard; Nasinyama, George WilliamThe study was conducted in the districts of Nebbi, Wakiso and Gomba in Uganda to characterize Diatomaceous Earth (DE) from the 3 major deposits in Uganda; explore status of exploitation and usage in agriculture, and anticipate associated effects. DE aka diatomite originated from fossilized remains of diatom shells. In agriculture, it’s used in controlling livestock internal and external parasites; post-harvest crop insect pests; and aflatoxins in stored feeds. DE has silica, Ca, Na, Mg, Fe, and other trace minerals making it valuable. Unlike synthetic drugs, DE is safer to consumers. Data collection employed both qualitative and quantitative approaches. Most respondents (94.6%) didn’t know of any agricultural DE usage, and the remaining (5.4%) knew it as a remedy against post-harvest weevils in stored grains. Residents in the mining area used DE in painting houses; craved by pregnant women; and relieving diarrhea in humans. Characterization revealed that Ugandan DE deposits were premium for various purposes. No commercial DE exploitation had started however when it’s due, the government would guide regulatory framework. Additionally, the socio-economic transformation was anticipated through employment creation; and foreign exchange. Since DE’s availability in Uganda is confirmed, more research and programs to promote its exploitation are needed.Item Efficacy of Diatomaceous Earth on Ascaridia galli, Blood Parameters: And on Ectoparasites In Chicken(Journal of Agricultural Science and Food Technology, 2019) Isabirye, Robert Alex; Waiswa, Charles; Kabi, Fred; Nanyeenya, William N.; Biryomumaisho, Savino; Acai-Okwee, James; Okello, Samuel; Omonuk Akello, Beatrice; Mwesigwa, Moses; Lumu, Richard; Nasinyama, George WilliamThe efficacy of diatomaceous earth (DE) in the treatment of chicken against Ascaridia galli and ectoparasites; and its effect on blood parameters in chicken was investigated. Four hundred hens were divided into 5 treatment groups, A, B, C, D and E (n = 80 per group). Groups C, D and E were orally infected with 250 embryonated A. galli eggs while groups A and B were not. Meanwhile, groups A and C fed diets with 2% DE and group D was fed with piperazine; and groups B and E was neither fed with DE nor piperazine. Fecal samples and blood samples were analyzed at week 16 to 22 and; 16 and 36, respectively for fecal egg counts (FEC) and blood parameters (erythrocyte count, hemoglobin content and hematocrit value), respectively. In another experiment, to assess efficacy of DE in treating poultry ectoparasites (fleas, mites and lice) DE was topically applied. Results from biweekly fecal analyses showed significant differences in FEC (P<0.05); and treatment by group (P<0.05) implying that DE had a significant effect in treating A. galli. No significant effects on hematological values were noted (P>0.05). Findings from ectoparasite trials indicated that at certain time points DE eliminated all targeted parasites (efficacy =100%). This study concludes that DE has the potential to control A. galli; and ectoparasites in chicken. Further studies should target effect of DE on internal parasite dynamics for longer periods in chicken. Additionally, to quicken the action of DE against ectoparasites, organic approaches should be studied.Item The Epidemiology of Zoonotic Brucellosis in Bahr el Ghazal Region of South Sudan(Frontiers in Public Health, 2019) Madut, Nuol Aywel; Muleme, James; Kankya, Clovice; Nasinyama, George William; Bwalya Muma, John; Godfroid, Jacques; Jubara, Ambrose Samuel; Muwonge, AdrianIn this study, we focused on three zoonotic brucellosis risk groups; abattoir workers, febrile cases at Wau hospital and cattle herders, in Bahr el Ghazal region, South Sudan. Competitive c-ELISA was used to detect anti-Brucella antibodies in 725 individuals between December 2015 andMay 2016. In addition, questionnairemetadata, focus group discussions and key informant interviews were used to characterize the epidemiology of zoonotic brucellosis in this region. Results: Overall, we estimate 27.2%(95%CI = 23.9–30.6) brucellosis sero-prevalence; 32.1% (95% CI = 26.2–38.4), 23.0% (95% CI = 19.1–27.4) and 34.6% (95% CI = 24.4–46.3) among abattoir workers, febrile cases, and herders, respectively. Marital status (Single, OR= 0.58, 95%CI: 0.36–0.91, P =0.02) and ethnicity (Kerash OR = 6.01, 95%CI: 1.97–21.10, P = 0.003 and Balanda, OR = 3.78, 95%CI: 1.42–12.02, P = 0.01) were associated with brucellosis. While gender and ethnicity were important factors for general awareness of zoonotic diseases. Highly ranked occupations at risk included veterinarian, butchers and milk handlers. We also identified covariate patterns for clinical diagnostics and public health interventions. Conclusion: We report the highest sero-prevalence of zoonotic brucellosis in three risk groups in the East African region. All this is not only occurring in a population with limited awareness that brucellosis is a zoonotic disease but also where one in nine health workers tested was sero-positive. We identified social demographic associations with brucellosis, however, the qualitative analysis suggests these are more complex and nuanced. Therefore, future studies could benefit from the use of the mixed methods approach to add extensiveness and depth to our understanding of zoonotic disease drivers, in order to implement mitigating measures such as cattle vaccination.Item Factors associated with severity and anatomical distribution of Diabetic Foot Ulcer in Uganda: A multicenter cross-sectional study(Research Square, 2022) Mumbere Vahwere, Bienfait; Ssebuufu, Robinson; Namatovu, Alice; Kyamanywa, Patrick; Ntulume, Ibrahim; Mugwano, Isaac; Theophilus, Pius; Katembo Sikakulya, Franck; Okedi, Francis Xaviour; Mulumba, Yusuf; Jorge, Soria; Agaba, Gidio; Nasinyama, George WilliamDiabetic foot ulcer (DFU) is a devastating complication of diabetes mellitus (DM) associated with high mortality and morbidity including amputations of lower extremities; and a high economic burden especially in low-income countries like Uganda. The aim of this study was to identify the factors associated with severity of DFU and assess the anatomical distribution of DFU among patients in Uganda. Methodology: This was a multicenter cross-sectional study conducted in 7 selected referral hospitals in Uganda. 117 patients with DM type 1 and 2 and foot ulcers were enrolled for this study from January to March 2021. Using the Wagner classification, patients were categorized as less severe DFU (grade 1 and grade 2) and severe DFU for grade 3 and above. A pre tested questionnaire was used to collect data. Data were analyzed using STATA Version 14 with significance at 95% and p-value of <0.05. Results: Out of 117 patients with DFU, 70 (59.8%) had severe DFU and 47 (40.2%) had less severe DFU. Mean age in years was 57.5 (SD15.2) among all study participants in general. The right foot was affected in (47.9%) of cases and the most frequent ulcer was found on the plantar of the foot (44.4%). Majority of the patients had one ulcer (50.4%) and the most frequent ulcer size was >5 cm (47.9%). Majority (61.5%) of the participants were female. Majority of participants, 90 (76.9%) also had uncontrolled blood sugars and 27(29.1%) had normal glycemia Severity of DFU was 3.4 more prevalent among patients with mild neuropathies (p=0.003), and 2.7 more prevalent for those with moderate neuropathies (p=0.005). Also, severity of DFU was 1.5 more prevalent in patients with an ulcer 5-10 cm of diameter (p=0.047) and 2.5 more prevalent in those with foot ulcer of more than 10 cm of diameter (p=.000). Conclusion:The study showed that most of the DFU patients have severe diabetic foot ulcer and uncontrolled glycemia. Neuropathies and ulcers more than 5cm wide are precipitating factors to severity of DFU and, therefore, early management is important to reduce the burden of the disease.Item Prevalence of brucellosis among patients attending Wau Hospital, South Sudan(PloS one, 2018) Madut, Nuol Aywel; Nasinyama, George William; Bwalya Muma, John; Sube, Kenneth L. L.; Ocan, Moses; Muwonge, Adrian; Godfroid, Jacques; Samuel Jubara, Ambrose; Kankya, CloviceBrucellosis is a zoonotic disease of public health importance; its prevalence varies globally. In low-income countries, brucellosis is an endemic and neglected disease affecting both animals and humans. This study was intended to establish brucellosis sero-prevalence among patients attending Wau hospital, South Sudan. Across sectional study, was done among randomly selected patients attending Wau hospital. Data was collected using questionnaires and laboratory investigations. Rose Bengal plate Test (RBPT), Serum agglutination test (SAT) and Competitive Enzyme Linked Immuno Sorbent Assay (c-ELISA) was used in the analysis of blood samples serially starting with RBPT which is more sensitive and least specific then SAT. c-ELISA test which is most specific and less sensitive compared to RBPT and SAT was then used to confirm presence of Brucella antibodies in the samples. A total of 416 participants out of 1664 were enrolled to this study. The majority of participants were between 7-to-76 years of age with mean age of 30.72 (SD+/- 12.83). The sero-positivity of patient's blood samples for brucellosis using c-ELISA was 23.3% (97/416) among patients presenting to Wau hospital. Socio-demographic characteristics, occupation, clinical signs of disease and types of animals reared by animal owners showed no significant correlation with occurrence of sero-positivity among patient's blood samples for brucellosis. While ethnicity (Nilotic), knowledge of zoonotic disease, and consumption of animal urine were statistically significant (p<0.05). The study found a high prevalence of brucellosis among febrile patients attending Wau hospital general outpatient clinic. There is need for co-ordination and collaboration between veterinary and health sectors of government to help prevent and control brucellosis in the region.Item Salmonella Species In Piglets And Weaners From Uganda:Prevalence, Antimicrobial Resistance And Herd-Level Riskfactors(Preventive veterinary medicine, 2014) Ikwap, Kokas; Erume, Joseph; Owiny, David Okello; Nasinyama, George William; Melin, Lennart; Bengtsson, Björn; Lundeheim, Nils; Fellström, Claes; Jacobson, MagdalenaNon-typhoidal salmonellosis is of concern in humans in sub-Saharan Africa, and this is partly due to the high number of immunocompromised persons. Pork and pork products could be among the sources of these non-typhi Salmonella spp. The aim of this study was to identify Salmonella spp. in piglets and weaners in northern and eastern Uganda, characterize their antimicrobial resistance patterns and determine herd-level risk factors. Fecal samples were collected from 465 piglets and weaners from 93 herds (49 and 44 from northern and eastern Uganda, respectively). In addition, information about the herd management and potential risk factors were collected. The fecal samples were cultured for the identification of Salmonella spp. The Salmonella spp. confirmed by serotyping were further characterized by determination of minimum inhibitory concentration (MIC) to 12 antimicrobials by broth microdilution. At individual level, the total prevalence of Salmonella spp. was 12% (12.2% in northern and 11.9% in eastern Uganda). At herd level, the total prevalence was 39% (43% in northern and 34% in eastern Uganda). From 56 samples with Salmonella spp., 20 serovars were identified including two serovars identified only by their antigenic formulae. The predominant serovars were S. Zanzibar, S. Heidelberg, S. Infantis, S. Typhimurium, S. Stanleyville, S. Aberdeen and S. Kampala. In total, 57% of the 53 Salmonella spp. analyzed, originating from 27% of the herds, were resistant to at least one antimicrobial agent. The majority of drug-resistant isolates (60%) were from northern Uganda. Eight multidrug-resistant (MDR) isolates were from northern Uganda and three MDR isolates were from eastern Uganda. Increased prevalence of Salmonella spp. was associated with feeding the young and adults separately as compared to feeding the young and adults together (p=0.043, OR=4.3; 95% CI 1.1, 17.38). Protective factors were “intensive” method of keeping the pigs versus “tethering and roaming” (p=0.016, OR=0.11; 95% CI 0.02, 0.64), “intensive” method versus “semi-intensive” method (p=0.048, OR=0.12; 95% CI 0.01, 0.96) and cleaning feeders after every two days versus daily (p=0.017, OR=0.18; 95% CI 0.05, 0.72). This study has revealed a high prevalence of infection of piglets and weaners with diverse non-typhi Salmonella serovars and highlights the potential role of pork and pork products as sources of these organisms for humans. In addition, this study has identified protective factors that could be promoted to control Salmonella spp. and in antimicrobial resistance reduction programs in rural pigs from Uganda.Item The sero-prevalence of brucellosis in cattle and their herders in Bahr el Ghazal region, South Sudan(PLOS Neglected Tropical Diseases, 2018) Madut, Nuol Aywel; Muwonge, Adrian; Nasinyama, George William; Bwalya Muma, John; Godfroid, Jacques; Jubara, Ambrose Samuel; Muleme, James; Kankya, CloviceBrucellosis is a worldwide recognized bacterial zoonotic disease. There is currently no information on bovine brucellosis sero-prevalence in South Sudan regardless of the economic, social and public health impact on populations. Therefore, for the first time in 33 years, we report the sero-prevalence of brucellosis in cattle and their herders. Furthermore, we characterize the drivers associated with the disease at the human-animal interface in Bahr el Ghazal region, South Sudan. Methods A total of 893 and 87 animal and human sera respectively were examined between December 2015 and May 2016. Rose Bengal Plate Test (RBPT) and Competitive Enzyme Linked Immuno Sorbent Assay (c-ELISA) were used in parallel to detect anti-Brucella antibodies. Questionnaires were administered to collect relevant metadata used for the association analysis in R version 3.2.3. Odds Ratio (OR) and Confidence Intervals (CI) were determined. Results Overall bovine brucellosis prevalence was 31% (95%CI = 28.0±34.2), with the highest 63% (95%CI = 53±70) and lowest 10% (95%CI = 4.5±20.1) prevalence estimates in Wau and Gogrial states respectively. The bovine sero-prevalence was approximately equally distributed among the male 30.4% (26.9±34.2) and the females 32.5% (26.8±38.7). Poor body condition (OR = 0.22; 95%CI = 0.07±0.54) and larger herd sizes (OR = 0.05; 95%CI = 0.008±0.173) were protective factors for brucellosis, while the opposite was true for the second (OR = 1.70; 95%CI = 1.08±2.67) and third (OR = 2.5; 95%CI = 1.46±4.47) lactation stage. The overall brucellosis sero-prevalence in herders was estimated at 33.3% (23.9± 44.3). Conclusion We report a high prevalence of anti-Brucella antibodies in cattle and their herders in Bahr el Ghazal, indicating an enzootic status in the cattle population being an important source of infection for humans. This represents a genuine public health challenge. Therefore, there is need to raise awareness and build capacity and infrastructure in this fragile state to underwrite future public health strategies for brucellosis.Item Seroprevalence And Risk Factors For Brucellosis In Cattle In Selected Districts Of Jimma Zone, Ethiopia(Tropical animal health and production, 2015) Dirar, Bashahun Gebremichael; Nasinyama, George William; Gelalcha, Benti DeresaA cross-sectional study was carried out in Jimma town and Chora Botor district of Jimma zone from February 2014 to May 2014 to determine seroprevalence and risk factors of brucellosis in cattle. A total of 348 blood samples (174 each from zebu and crossbreed) were collected. The sera were separated and screened by Rose Bengal plate test (RBPT), and positive sera were retested by complement fixation test (CFT) for confirmation. The overall seroprevalence of bovine brucellosis was 1.4 and 0.3 % as tested by RBPT and CFT, respectively. The seroprevalence of bovine brucellosis in indigenous and crossbreed cattle was 1.1 and 0.6 % and 1.7 and 0 % using RBPT and CFT, respectively. Retained fetal membrane was the only risk factor found to be significantly associated with seropositivity of brucellosis in this study (p = 0.019). The overall seroprevalence of brucellosis was very low. However, due to the zoonotic and economic importance of the disease, prevention and control measures are required to stop further spread of the disease. To effectively implement this, the One Health (OH) is the most constructive approach we recommend.