NRU :: Browsing by Author "Muyonga, John H."

Browsing by Author "Muyonga, John H."

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Bean-based nutrient-enriched puffed snacks: Formulation design, functional evaluation, and optimization
(Food Science & Nutrition, 2020) Muyonga, John H.; Nakimbugwe, Dorothy; Lung'aho, Mercy; Natabirwa, Hedwig; Tumwesigye, Kashub S.
School-age children frequently consume snacks. However, most of the snacks they consume are of low nutritional quality. The objective of this study was to develop a nutrient-rich and acceptable extruded bean-based snack, which could contribute to improved nutrient intake, especially for school-age children. Snack formulations developed from Roba1 beans, maize, orange-fleshed sweet potato, and amaranth mixtures, and processed in a twin-screw extruder, were evaluated and optimized for nutritional, textural and sensory properties. High proportion of beans in the formulation was associated with high protein, iron, zinc, and dietary fiber content. An optimal formulation (82.03:10: 5:2.97; beans, maize, OFSP, amaranth), containing 20.38 g, 4.12 g, 4.83 mg, and 1.51 mg per 100 g, of protein, dietary fiber, iron, and zinc, respectively, was obtained. The snacks were crunchy and moderately acceptable with average sensory scores of 6 on a 9-point hedonic scale, and hardness 26.6 N. Nutrient contribution 43, 19, and 12% for protein, iron, and zinc, respectively, to children aged 6 to 8 years; and 24, 19, and 7.6%, respectively, to children aged 9 to 12 years from a 40 g serving was estimated from the snack. The results demonstrate the potential of using extrusion to produce nutrient enriched value-added food products from blends of iron-rich beans and common staples.
Chemical and nutritional changes associated with the development of the hard-to-cook defect in common beans
(International Journal of Food Sciences and Nutrition, 2008) Nyakuni, Geoffrey A.; Kikafunda, Joyce K.; Muyonga, John H.; Kyamuhangire, William M.; Nakimbugwe, Dorothy; Ugen, Michael
Four common bean (Phaseolus vulgaris L.) varieties, Kawanda (K)131, K132, NABE4 and NABE11, were evaluated for the relationship between development of the hard-to-cook (HTC) defect and changes in nutritional quality during 6-month storage under ambient conditions. All varieties developed the HTC defect, but the extent was found to vary with variety. Cooking time increased by 113% in K131, 95.3% in K132, 56.4% in NABE4 and 42.93% in NABE11 after 6 months. The development of the HTC defect was found to be associated with a reduction in phytic acid content (r2 0.802), in vitro protein digestibility (r2 0.872) and in vitro starch digestibility (r2 0.729). The susceptibility to the HTC defect during storage could be attributed to a phytic acid interaction with proteins and carbohydrates, and is also associated with small seed size. Breeding for large seed size could therefore help reduce the development of the HTC defect.
Effect of heat processing on selected grain amaranth physicochemical properties
(Food science & nutrition, 2014) Muyonga, John H.; Andabati, Brian; Ssepuuya, Geoffrey
Grain amaranth is a pseudocereal with unique agricultural, nutritional, and functional properties. This study was undertaken to determine the effect of different heat-processing methods on physicochemical and nutraceutical properties in two main grain amaranth species, of Amaranthus hypochondriacus L. and Amaranthus cruentus L. Grains were prepared by roasting and popping, milled and analyzed for changes in in vitro protein digestibility, gruel viscosity, pasting characteristics, antioxidant activity, flavonoids, and total phenolics. In vitro protein digestibility was determined using the pepsin-pancreatin enzyme system. Viscosity and pasting characteristics of samples were determined using a Brookfield Viscometer and a Rapid Visco Analyzer, respectively. The grain methanol extracts were analysed for phenolics using spectrophotometry while antioxidant activity was determined using the DPPH (2,2-diphenyl-1-picrylhydrazyl) method. Heat treatment led to a reduction in protein digestibility, the effect being higher in popped than in roasted samples. Viscosities for roasted grain amaranth gruels were significantly higher than those obtained from raw and popped grain amaranth gruels. The results for pasting properties were consistent with the results for viscosity. In both A. hypochondriacus L. and A. cruentus L., the order of the viscosity values was roasted>raw>popped. The viscosities were also generally lower for A. cruentus L. compared to A. hypochondriacus L. Raw samples for both A. hypochondriacus L. and A. cruentus L. did not significantly differ in total phenolic content (TPC), total flavonoid content (TFC), and total antioxidant activity values. Thermal processing led to an increase in TFC and antioxidant activity. However, TPC of heat-processed samples remained unchanged. From the results, it can be concluded that heat treatment enhances antioxidant activity of grain amaranth and causes rheological changes dependent on the nature of heat treatment
Effect of processing methods on nutritional, sensory, and physicochemical characteristics of biofortified bean flour
(Food science & nutrition, 2016) Nkundabombi, Marie G.; Nakimbugwe, Dorothy; Muyonga, John H.
Common beans (Phaseolus vulgaris L.) are rich, nutritious and affordable by vulnerable groups, thus a good choice for biofortification to address malnutrition. However, increasing micronutrients content of beans, without improving micronutrients bioavailability will not improve the micronutrients status of consumers. Effect of different processing methods on the physicochemical characteristics of biofortified bean flour was determined. Processing methods used in this study were malting (48 h), roasting (170°C/45 min), and extrusion cooking using a twin screw extruder with three heating sections, the first set at 60°C, the second at 130°C, and the last one at 150°C. The screw was set at a speed of 35 Hz (123 g) and bean flour moisture content was 15%. Mineral extractability, in vitro protein digestibility, pasting properties, and sensory acceptability of porridge and sauce from processed flour were determined. All processing methods significantly increased (P < 0.05) mineral extractability, iron from 38.9% to 79.5% for K131 and from 40.7% to 83.4% for ROBA1, in vitro protein digestibility from 58.2% to 82% for ROBA1 and from 56.2% to 79% for K131. Pasting viscosities of both bean varieties reduced with processing. There was no significant difference (P < 0.05) between sensory acceptability of porridge or sauce from extruded biofortified bean flour and malted/roasted biofortified bean flour. Acceptability was also not affected by the bean variety used.
Effect of tamarind (Tamarindus indica L.) seed on antioxidant activity, phytocompounds, physicochemical characteristics, and sensory acceptability of enriched cookies and mango juice
(Food Science & Nutrition, 2016) Natukunda, Sheilla; Muyonga, John H.; Mukisa, Ivan M.
Tamarind seeds are not consumed despite their high antioxidative activity. In this study, 0–10% tamarind seed powder (TSP) was incorporated into mango juice and cookies. Total phenolics (Folin–Ciocalteu assay), antioxidant activity (2,2-diphenyl- 1 picrylhydrazyl (DPPH) radical scavenging assay), flavonoid (aluminum chloride assay), condensed tannins content (Vanillin-HCl assay), and consumer acceptability (n = 50) of the products were determined. TSP increased the pH and viscosity and reduced titratable acidity of juice. Incorporation of TSP increased the: total phenolic content (6.84 ± 0.21 to 88.44 ± 0.8 mg GAE/100 mL); flavonoid (4.64 ± 0.03–21.7 ± 0.36 mg CE/100 mL); condensed tannins (0.24 ± 0.01–21.81 ± 0.08 mg CE/100 mL) and total antioxidant activity (4.65 ± 0.88–21.70 ± 0.03 mg VCE/100 mL) of juice. A similar trend was observed for cookies. Maximum sensorially acceptable TSP levels were 1.5% and 6%, respectively, for juice and cookies. TSP can thus be utilized as a source of natural antioxidants in food products.
Effects of combined traditional processing methods on the nutritional quality of beans
(Food science & nutrition, 2015) Nakitto, Aisha M.; Muyonga, John H.; Nakimbugwe, Dorothy
Consumption of dry beans is limited by long cooking times thus high fuel requirement. The bioavailability of nutrients in beans is also limited due to presence of antinutrients such as phytates and tannins. Little research has been done on combined processing methods for production of nutritious fast cooking bean flour and the effect of combined treatments on nutritional quality of beans has not previously determined. The aim of this study was to reduce cooking time and enhance the nutritional value of dry beans. Specifically to: develop protocols for production of fast cooking bean flours and assess the effect of processing on the nutritional characteristics of the flours. Dry beans (K131 variety) were soaked for 12 h; sprouted for 48 h; dehulled and steamed for 25 and 15 min for whole and dehulled beans respectively or roasted at 170°C for 45 and 15 min for whole and dehulled beans respectively. Dehulling eliminated phytates and tannins and increased protein digestibility. In vitro protein digestibility and mineral (iron and zinc) extractability were negatively correlated with tannin and phytate content. Total available carbohydrates were highest in moist heat-treated bean flours. Overall, combined processing of beans improved the nutritional quality of dry beans and the resulting precooked flours need less cooking time compared to whole dry beans.
Exploring the Potential of Jackfruit (Artocarpus heterophyllus Lam)
(Asian Food Science Journal, 2021) Nansereko, Sophie; Muyonga, John H.
Jackfruit (Artocarpus heterophyllus Lam) is widely cultivated in tropical and subtropical areas worldwide. The tree is highly productive, resilient, and requires minimal care. Jackfruit has a characteristic flavour and is highly versatile. Several studies report the importance of the tree and fruits, ranging from food and medicine to the provision of several value-added products. In spite of the many benefits the plant offers, it has not been sufficiently incorporated in the general food system, partly because of gaps in processing, distribution and knowledge about utilization methods and nutritional and nutraceutical value. Objective: A comprehensive literature search was done to highlight jackfruit's nutritional, health, and commercial benefits to promote its utilization. Methodology: The literature search was conducted using three electronic databases with no constraints on publication year. Over 200 studies on the nutritional properties of jackfruit and at least 105 articles on the use of the various parts of the jack tree for their anticarcinogenic, antimicrobial, antifungal, anti-inflammatory, and hypoglycemic effects were found. Findings: Jackfruit has vital nutrients, including phytochemicals, minerals, vitamins, carbohydrates, and proteins. The literature on utilization shows that the fruit can be processed into commercial products using minimal processing technologies, dried to extend product shelf-life, or processed into various value-added products.
Factors influencing consumption of nutrient rich forest foods in rural Cameroon
(Appetite, 2016) Fungo, Robert; Snook, Laura; Muyonga, John H.; Kabahenda, Margaret; Okia, Clement A.
Studies show that a number of forest foods consumed in Cameroon are highly nutritious and rich in health boosting bioactive compounds. This study assessed the knowledge and perceptions towards the nutritional and health promoting properties of forest foods among forest dependent communities. The relationship between knowledge, perceptions and socio-demographic attributes on consumption of forest foods was also determined. A total of 279 females in charge of decision making with respect to food preparation were randomly selected from 12 villages in southern and eastern Cameroon and interviewed using researcher administered questionnaires. Multivariate logistic regression analysis was used to identify the factors affecting consumption of forest foods. Baillonella toxisperma (98%) and Irvingia gabonesis (81%) were the most known nutrient rich forest foods by the respondents. About 31% of the respondents were aware of the nutritional value and health benefits of forest foods. About 10%e61% of the respondents expressed positive attitudes to questions related with health benefits of specific forest foods. Consumption of forest foods was found to be higher among polygamous families and also positively related to length of stay in the forest area and age of respondent with consumption of forest foods. Education had an inverse relationship with use of forest foods. Knowledge and positive attitude towards the nutritional value of forest foods were also found to positively influence consumption of forest foods. Since knowledge was found to influence attitude and consumption, there is need to invest in awareness campaigns to strengthen the current knowledge levels among the study population. This should positively influence the attitudes and perceptions towards increased consumption of forest foods.
Fatty Acid Composition of Muscle, Liver, and Adipose Tissue of Freshwater Fish from Lake Victoria, Uganda
(Journal of Aquatic Food Product Technology, 2011) Masa, Justus; Ogwok, Patrick; Muyonga, John H.; Kwetegyeka, Justus; Makokha, Vincent; Ocen, Denis
Fish oils may differ in fatty acid (FA) composition depending on diet. Oils extracted from muscle, liver, and adipose tissue of Nile perch (Lates niloticus), Nile tilapia (Oreochromis niloticus), silver fish (Rastrineobola argentea), lungfish (Protopterus aethiopicus), Victoria squeaker (Synodontis victoriae), and two catfishes (Clarias gariepinus and Bagrus docmac) from Lake Victoria, a tropical freshwater lake, were evaluated for FA composition. Oil contents of muscles, livers, and adipose tissues were in the range of 3.16 to 13.8%, 3.62 to 53.4%, and 28.8 to 42.4%, respectively. Omega-3 polyunsaturated FA, particularly alpha-linolenic (ALA), eicosapentaenoic (EPA), docosapentaenoic (DPA), and docosahexaenoic (DHA) acids, were found to be in substantial amounts in oils from all seven fish species. Ratios of polyunsaturated FA to saturated FA (0.79 to 1.18) were in the range considered adequate for normal health. Overall, the results show that the fish species studied are a rich source of omega-3 polyunsaturated FA.
Fatty acid profile and stability of oil from the belly flaps of Nile perch (Lates niloticus)
(Food Chemistry, 2008) Ogwok, P.; Muyonga, John H.; Sserunjogi, M. L.
Oil extracted from the belly flaps of Lake Victoria Nile perch (Lates niloticus) was evaluated for fatty acid composition, contents of vitamin A, b-carotene and a-tocopherol, and oxidative stability. The oil was found to contain substantial amount of palmitic, palmitoleic, stearic, oleic, docosapentaenoic and docosahexaenoic fatty acids (FAs) and had high vitamin A content (3.94 ± 0.02 to 5.90 ± 0.02 mg/100 g of oil). Docosahexaenoic acid (10.45 ± 0.38%), docosapentaenoic acid (5.30 ± 0.60%) and eicosapentaenoic acid (3.63 ± 0.05%) were the most dominant polyunsaturated fatty acids (PUFAs). Ratios of PUFAs to saturated FAs were in the range 0.68 ± 0.02 to 0.74 ± 0.03, while the ratio of total x-3 FAs to total x-6 FAs was 0.85 ± 0.02 to 0.95 ± 0.08. The oils showed exceptional resistance to accelerated oxidation at 65 C probably because of its high content of b-carotene (2.93 ± 0.03 to 4.69 ± 0.01 mg/100 g of oil) and a-tocopherol (2.11 ± 0.03 to 11.4 ± 0.92 mg/100 g of oil). From the results, it can be concluded that Nile perch oil is a rich source of essential fatty acids and vitamin A.
Fruit and vegetable consumption, leisure-time physical activity, and sedentary behavior among children and adolescent students in Uganda
(Food Science & Nutrition, 2019) Ndagire, Catherine T.; Muyonga, John H.; Nakimbugwe, Dorothy
Introduction: Fruit and vegetable (FV) intake and participation in leisure-time physical activity (PA) and in sedentary behavior in accordance with the recommendations are met in a minority of populations including children and adolescents. Objective: To describe the frequency of FV intake, leisure-time PA, and sedentary behavior, assess compliance with international recommendations, and how selected factors are associated with these recommendations. Methods: The FV consumption and PA questionnaire items previously assessed for reliability in a Ugandan setting were used to gather information about FV consumption, PA, and sedentary behavior among 621 children and adolescents attending schools in Kampala Uganda. The results of FV consumption and PA were categorized as adequate or inadequate while sedentary behavior was categorized as adequate or excess. The relationship between FV consumption, PA, and sedentary behavior with selected independent variables was verified by chi-square test statistic. Results: Half of the participants (50%) reported inadequate fruit consumption, and 76% of them reported inadequate vegetable consumption. More children (68%) than young adolescents (52%) and older adolescents (44%) met their daily recommendation for fruit consumption. A similar trend was observed for vegetable consumption. Adequacy of FV intake was significantly associated with participant’s age, level of education, school ownership, and type of school. Less of 8-to 17-year- olds (14%) than 18 years and above olds (41%) met their weekly recommendation for leisure-time PA. Majority of participants (54%) participated in acceptable levels of sedentary behaviors. Leisure-time PA was significantly associated with age category and school ownership while sedentary behavior was significantly associated with participant’s age and education level. Conclusions: The intake of FV and participation in leisure-time PA was not adequate in the study population. A significant proportion of participants engaged in excess sedentary behavior. Developing and implementing strategies to increase FV intake and PA among children and adolescents in schools should be a national priority.
Microstructure and In Vitro Beta Carotene Bioaccessibility of Heat Processed Orange Fleshed Sweet Potato
(Plant foods for human nutrition, 2009) Tumuhimbise, Gaston A.; Namutebi, Agnes; Muyonga, John H.
Orange fleshed sweet potato (OFSP) has been identified as a good source of β-carotene but the β-carotene bio accessibility is affected by processing. In this study, the effect of traditional heat processing methods on the microstructure and in vitro bioaccessibility of β-carotene from OFSP were investigated. Bio accessibility was determined using simulated in vitro digestion model followed by membrane filtration to separate the micellar fraction containing bioaccessible β-carotene. Processing led to decrease in the amount of all-trans-β-carotene and increase in 13-cis-β- carotene. Processed OFSP had significantly higher (P<0.05) bioaccessible β-carotene compared to the raw forms. Bioaccessibility varied with processing treatments in the order; raw < baked < steamed/boiled < deep fried. Light microscopy showed that the microstructure of OFSP was disrupted by the processing methods employed. The cell walls of OFSP were sloughed by the traditional heat processing methods applied. The findings show that heat processing improves bioaccessibility of β-carotene in OFSP and this was probably due to
Morphological characteristics, bioactive compounds content, and antioxidant activity of different accessions of African eggplant (Solanum anguivi Lam.)
(J. Appl. Bot. Food Qual, 2021) Nakitto, Aisha Musaazi Sebunya; Byaruhanga, Yusuf Byenkya; Wagner, Anika E.; Muyonga, John H.
African eggplant (Solanum anguivi Lam.) fruits reportedly exhibit antidiabetic properties, possibly due to the presence of bioactive compounds. This study aimed to assess the bioactive compounds content (BCC) and antioxidant activity (AA) in the fruits of fourteen African eggplant accessions. The relationship between the fruit BCC and AA, and the plant (leaf, stem and fruit) morphological characteristics was determined. Morphological traits for the plant accessions were characterized based on existing Solanum species descriptors. Total phenolics, favonoids, saponins, vitamin C and AA were determined by spectrophotometry, while total alkaloids were detected by gravimetry. HPLC was used for the quantifcation of phenolic compounds. Morphological characteristics, BCC and AA differed among the accessions. The fruit’s accessions contained total phenolics (8.0-12.4 mg gallic acid equivalent/g dry weight (DW)), saponins (51.1-124.8 mg diosgenin equivalent/g DW), alkaloids (81.4-127.7 mg/g DW), vitamin C (3.6-6.4 mg ascorbic acid equivalent/g DW), and favonoids (0.9-2.1 mg quercetin equivalent (QE)/ g DW) and exhibited a high AA (1.2-4.6 mg QE/g DW). Amongst the quantifed phenolic compounds, chlorogenic acid (21.4-301.3 μg/ g DW) had the highest content. Cluster analyses showed that morphological characteristics might be useful to predict accessions with similar BCC and AA. Accessions with high total phenolics provided the highest AA, and, therefore, may mediate health benefts.
Nutrients and bioactive compounds content of Baillonella toxisperma, Trichoscypha abut and Pentaclethra macrophylla from Cameroon
(Food Science & Nutrition, 2015) Fungo, Robert; Muyonga, John H.; Kaaya, Archileo; Okia, Clement A.; Tieguhong, Juius C.; Baidu-Forson, Jojo J.
Baillonella toxisperma , Pentaclethra macrophylla and Trichoscypha abut are important foods for communities living around forests in Cameroon. Information on the nutritional value and bioactive content of these foods is required to establish their contribution to the nutrition and health of the communities. Samples of the three foods were obtained from four villages in east and three villages in south Cameroon. The foods were analyzed for proximate composition, minerals and bioactive content using standard chemical analysis methods. T. abut was found to be an excellent source of bioactive compounds; fl avonoids (306 mg/100 g), polyphenols (947 mg/100 g), proanthocyanins (61.2 mg/100 g), vitamin C (80.05 mg/100 g), and total oxalates (0.6 mg/100 g). P. macrophylla was found to be a rich source of total fat (38.71%), protein (15.82%) and total fi ber (17.10%) and some bioactive compounds; vitamin E (19.4 mg/100 g) and proanthocyanins (65.0 mg/100 g). B. toxisperma, was found to have high content of carbohydrates (89.6%), potassium (27.5 mg/100 g) and calcium (37.5 mg/100 g). Flavonoids, polyphenols, vitamins C and E are the main bioactive compounds in these forest foods. The daily consumption of some of these fruits may coffer protection against some ailments and oxidative stress. Approximately 200 g of either B. toxisperma or P. macrophylla, can supply 100% iron and zinc RDA s for children aged 1–3 years, while 300 g of the two forest foods can supply about 85% iron and zinc RDA s for non- pregnant nonlactating women. The three foods provide 100% daily vitamins C and E requirements for both adults and children. The results of this study show that Baillonella toxisperma , Pentaclethra macrophylla and Trichoscypha abut can considerably contribute towards the human nutrient requirements. These forest foods also contain substantial levels of health promoting phytochemicals notably fl avonoids, polyphenols, vitamins C and E. These foods therefore have potential to promote nutrition and health, especially among forest dependent communities who consume them in substantial amounts.
Optimized formulation and processing protocol for a supplementary bean-based composite flour
(Food Science & Nutrition, 2015) Ndagire, Catherine T.; Muyonga, John H.; Manju, Reddy; Nakimbugwe, Dorothy
Protein-energy malnutrition is the most serious nutritional body depletion disorder among infants and young children in developing countries, attributable to inadequate energy and nutrient intake, partly due to high dietary bulk of weaning and infant foods. The gruels fed to children are typically of low nutrient and energy density due to the low flour incorporation rate required for drinking viscosity. The aim of this study was to develop a nutritious product, based on common dry beans and other grains, suitable for supplementary feeding. The optimal processing conditions for desired nutritional and sensory attributes were determined using Response Surface Methodology. For bean processing, soaking for 6, 15, or 24 h, germination for 24 or 48 h, and cooking under pressure for either 10 or 20 min were the independent variables. The processed bean flour’s total polyphenol, phytic acid and protein content, the sensory acceptability of the bean-based composite porridge and its protein and starch digestibility were dependent variables. Based on product acceptability, antinutrients and protein content, as well as on protein and starch digestibility, the optimum processing conditions for the bean flour for infant and young child feeding were 24 h of soaking, 48 h of malting, and 19 min of steaming under pressure. These conditions resulted in a product with the highest desirability. The model equations developed can be used for predicting the quality of the bean flour and the bean-based composite porridge. Bean optimally processed and incorporated with grain amaranth and rice flours of a ratio of 40: 30: 30, respectively, resulted into flour with high energy, mineral, and nutrient density of the final porridge. The composite is well adaptable to preparation at rural community level. The use of these locally available grains and feasible processes could make a great contribution to nutrition security in sub-Saharan Africa and other developing countries.
Pesticide Residues and Heavy Metals in Lake Victoria Nile Perch, Lates niloticus, Belly Flap Oil
(Bulletin of environmental contamination and toxicology, 2009) Ogwok, P.; Muyonga, John H.; Sserunjogi, M. L.
Oil was extracted from the belly flaps of varied sizes of Nile perch caught from Lake Victoria (Uganda). The oil was analyzed for pesticide residues and heavy metals. Total residual concentration of dichlorodiphenyltrichloroethane, endosulfan, hexachlorocyclohexane, hexachlorobenzene, heptachlor, chlordane, endrin, aldrin and chlorofenvinphos increased significantly (p\0.05) with fish size. Mercury and lead were detected in most samples while arsenic and cadmium were below detection limits. Nile perch may, therefore, accumulate significant amount of chemical contaminants. Levels of contaminants in Nile perch oil were, in general, within limits considered acceptable by the stringent German Food Law for human consumption.
Physicochemical Characteristics of Yam Bean (Pachyrhizus erosus) Seed Proteins
(Journal of Food Research, 2014) Kisambira, Abbas; Muyonga, John H.; Byaruhanga, Yusuf B.; Tukamuhabwa, Phinehas; Tumwegamire, Silver; Gruenberg, Wolfgang
This study sought to determine the physicochemical and functional properties of yam bean (Pachyrhizus erosus) seed proteins. Pachyrhizus erosus seeds from two accessions (UYB 06 and UYB 07) were milled into flours and then defatted. A portion of the defatted flour was used for production of protein isolates and protein fractions. The physicochemical and functional properties, in vitro digestibility and electrophoretic pattern of the flour and protein isolate were determined. The results showed that albumins (53.3%) were the dominant protein fraction followed by globulins (18.7%), glutelins (8.8%) and prolamins (2.7%). Regarding functional properties, the Pachyrhizus erosus seed protein isolates exhibited 8% of least gelation concentration, water absorption capacity of 3.0 g g-1, oil absorption capacity of 0.8 g g-1, protein solubility of 81.0%, foaming capacity of 37.1%, foam stability of 73.8%, emulsion activity of 13.8% and emulsion stability of 9.2%. In vitro protein digestibility of the raw and cooked beans was 87.6% and 84.3%, respectively. The electrophoretic pattern of Pachyrhizus erosus protein showed major bands corresponding to molecular weight 13.3, 15, 29.8, 54.4 and above 84.7 kDa. The results, suggest that Pachyrhizus erosus seed protein has potential for use in both food and non-food applications such as films and coating.
Prediction equations for body composition of children and adolescents aged 8e19 years in Uganda using deuterium dilution as the reference technique
(Clinical Nutrition ESPEN, 2018) Ndagire, Catherine T.; Muyonga, John H.; Odur, Benard; Nakimbugwe, Dorothy
Background and aims: Body composition is important as a marker of both current and future health status. Bioelectrical impedance analysis (BIA) is a simple and accurate method for estimating body composition in field, clinical and research settings, if standard protocol procedures are followed. However, BIA requires population-specific equations since applicability of existing equations to diverse populations has been questioned. This study aimed to derive predictive equations for Total Body Water (TBW), Fat Free Mass (FFM) and Fat Mass (FM) determinations with BIA and anthropometric measurements in a population of children and adolescents aged 8e19 years in Uganda. Methods: A cross-sectional study was conducted among 203 children and adolescents aged 8e19 years attending schools in Kampala district (also referred to as Kampala city since the city is conterminous with the district), Uganda through a two-stage cluster sample design. Deuterium dilution method (DDM) was used as the reference measure while BIA and anthropometric measures were used to create the new body composition prediction equations through multivariate regression. Results: The new prediction equations explained 88%, 87% and 71% of the variance in TBW, FFM % and of FM respectively with no statistical shrinkage upon cross-validation. The linear regression models proposed in this study were well adjusted with respect to TBW, FFM and FM. Log of TBW obtained by DDM ¼ 0.0129 Impedance index þ 0.0055 Age þ 0.0049Waist Circumference þ 0.1219Ht2 þ 2.0388. Log of FFM obtained by DDM ¼ 0.0197 FFM obtained by BIA e 0.0181 sex code e 0.00055 Impedance þ 3.1761. Log of FM obtained by DDM ¼ 0.0634 FM obtained by BIA e 0.1881 sex code þ 0.0252 Weight þ 0.5273.
Solanum anguivi Lam. Fruits: Their Potential Effects on Type 2 Diabetes Mellitus
(Molecules, 2021) Nakitto, Aisha Musaazi Sebunya; Muyonga, John H.; Byaruhanga, Yusuf Byenkya; Wagner, Anika E.
Type 2 diabetes mellitus (T2DM) is a complex metabolic disorder of glucose homeostasis associated with a status of insulin resistance, impaired insulin signaling, β-cell dysfunction, impaired glucose and lipid metabolism, sub-clinical inflammation, and increased oxidative stress. Consuming fruits and vegetables rich in phytochemicals with potential antidiabetic effects may prevent T2DM and/or support a conservative T2DM treatment while being safer and more affordable for people from low-income countries. Solanum anguivi Lam. fruits (SALF) have been suggested to exhibit antidiabetic properties, potentially due to the presence of various phytochemicals, including saponins, phenolics, alkaloids, ascorbic acid, and flavonoids. For the saponin fraction, antidiabetic effects have already been reported. However, it remains unclear whether this is also true for the other phytochemicals present in SALF. This review article covers information on glucose homeostasis, T2DM pathogenesis, and also the potential antidiabetic effects of phytochemicals present in SALF, including their potential mechanisms of action.
Validity and Reliability of General Nutrition Knowledge Questionnaire for Adults in Uganda
(Nutrients,, 2017) Bukenya, Richard; Ahmed, Abhiya; Andrade, Jeanette M.; Grigsby-Toussaint, Diana S.; Muyonga, John H.; Andrade, Juan E.
This study sought to develop and validate a general nutrition knowledge questionnaire (GNKQ) for Ugandan adults. The initial draft consisted of 133 items on five constructs associated with nutrition knowledge; expert recommendations (16 items), food groups (70 items), selecting food (10 items), nutrition and disease relationship (23 items), and food fortification in Uganda (14 items). The questionnaire validity was evaluated in three studies. For the content validity (study 1), a panel of five content matter nutrition experts reviewed the GNKQ draft before and after face validity. For the face validity (study 2), head teachers and health workers (n = 27) completed the questionnaire before attending one of three focus groups to review the clarity of the items. For the construct and test-rest reliability (study 3), head teachers (n = 40) from private and public primary schools and nutrition (n = 52) and engineering (n = 49) students from Makerere University took the questionnaire twice (two weeks apart). Experts agreed (content validity index, CVI > 0.9; reliability, Gwet’s AC1 > 0.85) that all constructs were relevant to evaluate nutrition knowledge. After the focus groups, 29 items were identified as unclear, requiring major (n = 5) and minor (n = 24) reviews. The final questionnaire had acceptable internal consistency (Cronbach > 0.95), test-retest reliability (r = 0.89), and differentiated (p < 0.001) nutrition knowledge scores between nutrition (67 5) and engineering (39 11) students. Only the construct on nutrition recommendations was unreliable (Cronbach = 0.51, test-retest r = 0.55), which requires further optimization. The final questionnaire included topics on food groups (41 items), selecting food (2 items), nutrition and disease relationship (14 items), and food fortification in Uganda (22 items) and had good content, construct, and test-retest reliability to evaluate nutrition knowledge among Ugandan adults.

Browsing by Author "Muyonga, John H."

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