Browsing by Author "Magoma, Gabriel"

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    Advances in green nanobiotechnology: Data for synthesis and characterization of silver nanoparticles from ethanolic extracts of fruits and leaves of Annona muricata
    (Data in brief, 2019) Gavamukulya, Yahaya; El-Shemy, Hany A.; Meroka, Amos M.; Madivoli, Edwin S.; Maina, Esther N.; Wamunyokoli, Fred; Magoma, Gabriel
    In this data article, data obtained from an efficient, eco-friendly and low-cost method for the synthesis and recovery of Silver nanoparticles (AgNPs) using ethanolic extracts of Annona muricata fruits and leaves as reducing, stabilizing and capping agents has been reported. 99.7% pure silver nitrate was used as the inorganic ion source. The data was obtained using different spectroscopic and microscopic techniques. The data is presented in form of images, Microsoft excel sheets, graphs,.raw files,.dpt files, PDF files, among others. Methods of analysis and interpretation of the data have also been presented. The data can be most useful to researchers, research students, industrialists and academicians to acquire knowledge on the green synthesis of AgNPs and related
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    Annona muricata silver nanoparticles exhibit strong anticancer activities against cervical and prostate adenocarcinomas through regulation of CASP9 and the CXCL1/CXCR2 genes axis
    (Tumor Biology, 2021) Gavamukulya, Yahaya; Maina, Esther N.; El-Shemy, Hany A.; Meroka, Amos M.; Kangogo, Geoffrey K.; Magoma, Gabriel; Wamunyokoli, Fred
    Green synthesized nanoparticles have been earmarked for use in nanomedicine including for the development of better anticancer drugs. OBJECTIVE: The aim of this study was to undertake biochemical evaluation of anticancer activities of green synthesized silver nanoparticles (AgNPs) from ethanolic extracts of fruits (AgNPs-F) and leaves (AgNPs-L) of Annona muricata. METHODS:Previously synthesized silver nanoparticles were used for the study. The effects of the AgNPs and 5-Fluorouracil were studied on PC3, HeLa and PNT1A cells. The resazurin, migration and colonogenic assays as well as qRT-PCR were employed. RESULTS: The AgNPs-F displayed significant antiproliferative effects against HeLa cells with an IC50 of 38.58 g/ml and PC3 cells with an IC50 of 48.17 g/ml but selectively spared normal PNT1A cells (selectivity index of 7.8), in comparison with first line drug 5FU and AgNPs-L whose selectivity index were 3.56 and 2.26 respectively. The migration assay revealed potential inhibition of the metastatic activity of the cells by the AgNPs-F while the colonogenic assay indicated the permanent effect of the AgNPs-F on the cancer cells yet being reversible on the normal cells in contrast with 5FU and AgNPs-L. CASP9 was significantly over expressed in all HeLa cells treated with the AgNPs-F (1.53-fold), AgNPs-L (1.52-fold) and 5FU (4.30-fold). CXCL1 was under expressed in HeLa cells treated with AgNPs-F (0.69-fold) and AgNPs-L (0.58-fold) and over expressed in cells treated with 5FU (4.95-fold), but the difference was not statistically significant. CXCR2 was significantly over expressed in HeLa cells treated with 5FU (8.66-fold) and AgNPs-F (1.12-fold) but under expressed in cells treated with AgNPs-L (0.76-fold). CONCLUSIONS: Here we show that biosynthesized AgNPs especially AgNPs-F can be used in the development of novel and better anticancer drugs. The mechanism of action of the AgNPs involves activation of the intrinsic apoptosis pathway through upregulation of CASP9 and concerted down regulation of the CXCL1/ CXCR2 gene axis.
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    Green Synthesis and Characterization of Highly Stable Silver Nanoparticles from Ethanolic Extracts of Fruits of Annona muricata
    (Journal of Inorganic and Organometallic Polymers and Materials, 2020) Gavamukulya, Yahaya; Maina, Esther N.; Meroka, Amos M.; Madivoli, Edwin S.; El‑Shemy, Hany A.; Wamunyokoli, Fred; Magoma, Gabriel
    Green synthesis of nanoparticles from plant materials opens a new scope in nanobiotechnology and discourages the use of expensive toxic chemicals. The aim of this study was to develop and optimize a method for the synthesis of Silver Nanoparticles (AgNPs) from ethanolic extracts of fruits of Annona muricata as well as to characterize the green synthesized AgNPs. AgNPs were synthesized via AgNO3 solution. The AgNPs were characterized using spectroscopy and microscopy techniques. The formed AgNPs had an absorption maximum of 427 nm and were stable under different temperature, pH and storage conditions. Fourier Transform Infrared Resorption spectroscopy revealed the functional groups responsible for the synthesis and stabilization of the AgNPs. Scanning Electron Microscopy analysis revealed a spherical nature of the AgNPs. Energy Dispersive X-Ray spectroscopy showed presence of Ag, Cl, Ca, and Si with Ag having the highest composition at 80%. X-ray diffraction and dynamic light scattering revealed a crystalline nature of AgNPs with an average size of 60.12 nm and a polydispersity index of 0.1235 respectively. Transmission Electron Microscopy analysis further confirmed the crystalline and spherical nature of the AgNPs. In this article, an efficient, eco-friendly and low-cost method for the synthesis and recovery of stable AgNPs using ethanolic extracts of Annona muricata fruits as both reducing and capping agents has been reported. The synthesized AgNPs could have many biomedical and clinical applications.
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    In search of new anticancer drugs: Data for cytotoxic activities of green synthesized silver nanoparticles from ethanolic extracts of fruits and leaves of Annona muricata and 5- Fluorouracil against HeLa, PC3 and PNT1A cell lines
    (Data in brief, 2019) Gavamukulya, Yahaya; Maina, Esther N.; Meroka, Amos M.; El-Shemy, Hany A.; Magoma, Gabriel; Wamunyokoli, Fred
    In this article, we present data on the anticancer activities of green synthesized silver nanoparticles (AgNPs) from ethanolic extracts of fruits (AgNPs-F) and leaves (AgNPs-L) of Annona muricata and standard anticancer drug 5-Fluorouracil (5-FU) on two cancer cell lines, i.e. cervical adenocarcinoma (HeLa cells) and prostate adenocarcinoma (PC3 cells) as well as on an immortalized normal prostate cell line, PNT1A. The cytotoxicity on the cells was determined by measuring the absorbance signal of resazurin dye. It has long been known that metabolically active cells change the resazurin from blue (oxidized) to red (reduced) forms, corresponding to the absorbance signals at a wavelength of 570nm (A570) and 600nm (A600) respectively, from which therefore the effects of any treatments on percentage cell viability/death can be elucidated. The raw data values of the treatments against the HeLa, PC3 and PNT1A cells are shown in the different Tables. Examples of how the data can be analyzed have been illustrated using different growth inhibition curves. The data can be used by academics, students, and researchers working on development of anticancer drugs.
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    Liquid Chromatography Single Quadrupole Mass Spectrometry (LC/SQ MS) Analysis Reveals Presence of Novel Antineoplastic Metabolites in Ethanolic Extracts of Fruits and Leaves of Annona muricata
    (Pharmacognosy Journal, 2019) Gavamukulya, Yahaya; Maina, Esther N.; Meroka, Amos M.; Madivoli, Edwin S.; El-Shemy, Hany A.; Magoma, Gabriel; Wamunyokoli, Fred
    Annona muricata, a tropical plant species belonging to family Annonaceae is one of the most used plants in folk medicine because of its many medicinal uses. Despite its wide usage, there is still need to continue scientifically evaluating its medicinal properties in order to avoid any adverse effects. Elucidating the detailed chemical composition of this plant is a significant step towards this evaluation. Objective: The aim of this study was to conduct LC MS analysis on the ethanolic extracts of fruits and leaves of Annona muricata for detection of novel metabolites. Materials and Methods: Leaves and fruits of Annona muricata were collected from Eastern Uganda during the month January 2018. Extraction was conducted using the tissue homogenization method and the extracts were analyzed on an LC/SQ MS detection system. The results were obtained by analyzing the MS spectra using the retentions time and fragmentation patterns on the NIST Library. Results: The study revealed that the fruits extracts contain 1,3-Dimethylthiourea and (4-chlorophenyl)-[4-(3-chlorophenyl)-2-[(Z)-3-(dimethylamino) prop-1-enyl]quinolin-6-yl]-(3-methylimidazol-4-yl)methanol, which are reported antioxidant and antineoplastic agents. The leaves contained 2,4,6-Tribromoaniline another antioxidant and antineoplastic agent, while compound (dichlorozirconium(2+);dimethyl-bis(2-methyl-4- phenylinden-1-id-1-yl)silane was found in both extracts of fruits and leaves. Conclusion: The current study suggests that ethanolic extracts of fruits and leaves of Annona muricata contain compounds which are potent antioxidant, antineoplastic and therapeutic agents for various conditions and paves the way for the development of several treatment regimens from these plant parts. Finally, the compounds reported in this study have been identified for the first time as being found in Annona muricata.
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    Synthesis and Characterization of Silver Nanoparticles from Ethanolic Extracts of Leaves of Annona muricata: A Green Nanobiotechnology Approach
    (Biotechnology Journal International, 2019) Gavamukulya, Yahaya; Maina, Esther N.; Wamunyokoli, Fred; Meroka, Amos M.; Madivoli, Edwin S.; El-Shemy, Hany A.; Magoma, Gabriel
    The biological green synthesis of nanoparticles via nanobiotechnology processes have a significant potential to boost nanoparticles production without the use of harsh, toxic, and expensive chemicals commonly used in conventional physical and chemical processes. Annona muricata, a tropical plant belonging to family Annonaceae is one of the most used plants in folk medicine because of its many medicinal uses and therefore presents a strong candidate for use in green synthesis. Aims: The aim of this study was to optimize a method for the synthesis of Silver Nanoparticles (AgNPs) from ethanolic extracts of leaves of Annona muricata as well as to characterize the green synthesized AgNPs. Methodology: AgNPs were synthesized from Annona muricata leaves using AgNO3 solution. The AgNPs were characterized using spectroscopy and microscopy techniques. Results: The formed AgNPs had an absorption maximum at 429 nm using UV–Visible spectroscopy and were stable under different pH, temperature, and storage conditions. Fourier transform infrared analysis revealed the different functional groups responsible for the synthesis and stabilization of the AgNPs. Scanning electron microscopy analysis revealed a spherical nature of the synthesized AgNPs. Energy dispersive x-ray spectroscopy analysis showed presence of Ag, O, and Cl with Ag having the highest composition at 60%. X-Ray Diffraction and Dynamic Light Scattering revealed a crystalline nature of AgNPs with an average size of 87.36 nm and a polydispersity index of 0.16 respectively. Transmission Electron Microscopy analysis further confirmed the crystalline and spherical nature of the AgNPs. Conclusion: In this article, an efficient, eco-friendly and low-cost method for the synthesis and recovery of stable AgNPs using Annona muricata leaves ethanolic extracts as both a reducing and capping agent has been reported for the first time. The synthesized AgNPs could be promising candidates for many biomedical, clinical, engineering, and polymer applications.